Abstract
The purpose of this study was to evaluate the effect of aging (12 vs. 24 mo) on skeletal muscle insulin receptor function of male Sprague-Dawley rats fed either a 33% sucrose (wt/wt) or sucrose-free diet. The effect of exercise in combination with the sucrose diet was also evaluated by exercising half of the sucrose-fed group on a motorized treadmill. Insulin-receptor function was assessed in vitro by measuring the binding capacity of [125I]-insulin to partially purified receptors of the biceps femoris and vastus lateralis. Tyrosine kinase activity was measured as an index of postreceptor function. Insulin-receptor number was significantly decreased in 24-mo-old sucrose-fed rats compared to 12-mo-old rats fed the sucrose or sucrose-free diets. The affinity of insulin for the receptor did not significantly differ among groups. Maximal tyrosine kinase activity in vastus lateralis was significantly decreased in 12-mo-old sucrose-fed rats compared with sedentary 24-mo-old rats fed the sucrose-free diet or 24-mo-old rats fed the sucrose diet in combination with exercise. Exercise prevented the decrease in receptor function in both 12- and 24-mo-old sucrose-fed rats as measured by insulin binding and tyrosine kinase activity. These data suggest that diet and/or exercise rather than aging per se has a greater influence on insulin-receptor function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1081-1089 |
| Number of pages | 9 |
| Journal | Journal of Nutrition |
| Volume | 121 |
| Issue number | 7 |
| State | Published - 1991 |
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Keywords
- Aging
- Insulin binding
- Insulin resistance
- Rats
- Skeletal muscle
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Food Science
Cite this
High sucrose diet and exercise : Effects on insulin-receptor function of 12- and 24-mo-old Sprague-Dawley rats. / Eiffert, K. C.; McDonald, R. B.; Stern, J. S.
In: Journal of Nutrition, Vol. 121, No. 7, 1991, p. 1081-1089.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High sucrose diet and exercise
T2 - Effects on insulin-receptor function of 12- and 24-mo-old Sprague-Dawley rats
AU - Eiffert, K. C.
AU - McDonald, R. B.
AU - Stern, J. S.
PY - 1991
Y1 - 1991
N2 - The purpose of this study was to evaluate the effect of aging (12 vs. 24 mo) on skeletal muscle insulin receptor function of male Sprague-Dawley rats fed either a 33% sucrose (wt/wt) or sucrose-free diet. The effect of exercise in combination with the sucrose diet was also evaluated by exercising half of the sucrose-fed group on a motorized treadmill. Insulin-receptor function was assessed in vitro by measuring the binding capacity of [125I]-insulin to partially purified receptors of the biceps femoris and vastus lateralis. Tyrosine kinase activity was measured as an index of postreceptor function. Insulin-receptor number was significantly decreased in 24-mo-old sucrose-fed rats compared to 12-mo-old rats fed the sucrose or sucrose-free diets. The affinity of insulin for the receptor did not significantly differ among groups. Maximal tyrosine kinase activity in vastus lateralis was significantly decreased in 12-mo-old sucrose-fed rats compared with sedentary 24-mo-old rats fed the sucrose-free diet or 24-mo-old rats fed the sucrose diet in combination with exercise. Exercise prevented the decrease in receptor function in both 12- and 24-mo-old sucrose-fed rats as measured by insulin binding and tyrosine kinase activity. These data suggest that diet and/or exercise rather than aging per se has a greater influence on insulin-receptor function.
AB - The purpose of this study was to evaluate the effect of aging (12 vs. 24 mo) on skeletal muscle insulin receptor function of male Sprague-Dawley rats fed either a 33% sucrose (wt/wt) or sucrose-free diet. The effect of exercise in combination with the sucrose diet was also evaluated by exercising half of the sucrose-fed group on a motorized treadmill. Insulin-receptor function was assessed in vitro by measuring the binding capacity of [125I]-insulin to partially purified receptors of the biceps femoris and vastus lateralis. Tyrosine kinase activity was measured as an index of postreceptor function. Insulin-receptor number was significantly decreased in 24-mo-old sucrose-fed rats compared to 12-mo-old rats fed the sucrose or sucrose-free diets. The affinity of insulin for the receptor did not significantly differ among groups. Maximal tyrosine kinase activity in vastus lateralis was significantly decreased in 12-mo-old sucrose-fed rats compared with sedentary 24-mo-old rats fed the sucrose-free diet or 24-mo-old rats fed the sucrose diet in combination with exercise. Exercise prevented the decrease in receptor function in both 12- and 24-mo-old sucrose-fed rats as measured by insulin binding and tyrosine kinase activity. These data suggest that diet and/or exercise rather than aging per se has a greater influence on insulin-receptor function.
KW - Aging
KW - Insulin binding
KW - Insulin resistance
KW - Rats
KW - Skeletal muscle
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UR - http://www.scopus.com/inward/citedby.url?scp=0025914530&partnerID=8YFLogxK
M3 - Article
C2 - 2051228
AN - SCOPUS:0025914530
VL - 121
SP - 1081
EP - 1089
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 7
ER -