High-resolution voxelation mapping of human and rodent brain gene expression

Ram P. Singh, Vanessa M. Brown, Abhijit Chaudhari, Arshad H. Khan, Alex Ossadtchi, Daniel M. Sforza, A. Ken Meadors, Simon R Cherry, Richard M. Leahy, Desmond J. Smith

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Voxelation allows high-throughput acquisition of multiple volumetric images of brain gene expression, similar to those obtained from biomedical imaging systems. To obtain these images, the method employs analysis of spatially registered voxels (cubes). For creation of high-resolution maps using voxelation, relatively small voxel sizes are necessary and instruments will be required for semiautomated harvesting of such voxels. Here, we describe two devices that allow spatially registered harvesting of voxels from the human and rodent brain, giving linear resolutions of 3.3 and 1 mm, respectively. Gene expression patterns obtained using these devices showed good agreement with known expression patterns. The voxelation instruments and their future iterations represent a valuable approach to the genome scale acquisition of gene expression patterns in the human and rodent brain.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalJournal of Neuroscience Methods
Volume125
Issue number1-2
DOIs
StatePublished - May 30 2003

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Rodentia
Gene Expression
Brain
Equipment and Supplies
Genome

Keywords

  • Brain mapping
  • Expression profiling
  • Functional genomics
  • Microarrays
  • Photoetching
  • Voxelation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Singh, R. P., Brown, V. M., Chaudhari, A., Khan, A. H., Ossadtchi, A., Sforza, D. M., ... Smith, D. J. (2003). High-resolution voxelation mapping of human and rodent brain gene expression. Journal of Neuroscience Methods, 125(1-2), 93-101. https://doi.org/10.1016/S0165-0270(03)00045-1

High-resolution voxelation mapping of human and rodent brain gene expression. / Singh, Ram P.; Brown, Vanessa M.; Chaudhari, Abhijit; Khan, Arshad H.; Ossadtchi, Alex; Sforza, Daniel M.; Meadors, A. Ken; Cherry, Simon R; Leahy, Richard M.; Smith, Desmond J.

In: Journal of Neuroscience Methods, Vol. 125, No. 1-2, 30.05.2003, p. 93-101.

Research output: Contribution to journalArticle

Singh, RP, Brown, VM, Chaudhari, A, Khan, AH, Ossadtchi, A, Sforza, DM, Meadors, AK, Cherry, SR, Leahy, RM & Smith, DJ 2003, 'High-resolution voxelation mapping of human and rodent brain gene expression', Journal of Neuroscience Methods, vol. 125, no. 1-2, pp. 93-101. https://doi.org/10.1016/S0165-0270(03)00045-1
Singh, Ram P. ; Brown, Vanessa M. ; Chaudhari, Abhijit ; Khan, Arshad H. ; Ossadtchi, Alex ; Sforza, Daniel M. ; Meadors, A. Ken ; Cherry, Simon R ; Leahy, Richard M. ; Smith, Desmond J. / High-resolution voxelation mapping of human and rodent brain gene expression. In: Journal of Neuroscience Methods. 2003 ; Vol. 125, No. 1-2. pp. 93-101.
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