High-resolution three-dimensional imaging of the rich membrane structures of bone marrow-derived mast cells

T. Zink, Z. Deng, H. Chen, L. Yu, Fu-Tong Liu, Gang-yu Liu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Atomic force microscopy (AFM) enables high-resolution three-dimensional (3D) imaging of cultured bone marrow-derived mast cells. Cells were immobilized by a quick centrifugation and fixation to preserve their transient cellular morphologies followed by AFM characterization in buffer. This "fix-and-look" approach preserves the structural integrity of individual cells. Well-known membrane morphologies, such as ridges and microvilli, are visualized, consistent with prior electron microscopy observations. Additional information including the 3D measurements of these characteristic features are attained from AFM topographs. Filopodia and lamellopodia, associated with cell spreading, were captured and visualized in three dimensions. New morphologies are also revealed, such as high-density ridges and micro-craters. This investigation demonstrates that the "fix-and-look" approach followed by AFM imaging provides an effective means to characterize the membrane structure of hydrated cells with high resolution. The quantitative imaging and measurements pave the way for systematic correlation of membrane structural features with the biological status of individual cells.

Original languageEnglish (US)
Pages (from-to)22-31
Number of pages10
JournalUltramicroscopy
Volume109
Issue number1
DOIs
StatePublished - Dec 2008

Fingerprint

membrane structures
Membrane structures
bone marrow
Atomic force microscopy
Bone
Imaging techniques
atomic force microscopy
high resolution
cells
fixing
ridges
Membranes
Centrifugation
membranes
Structural integrity
Electron microscopy
Buffers
craters
integrity
electron microscopy

Keywords

  • Atomic force microscopy
  • Bone marrow-derived mast cells
  • Cell membrane
  • Craters
  • High-resolution imaging
  • Ridges
  • Villi

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electronic, Optical and Magnetic Materials

Cite this

High-resolution three-dimensional imaging of the rich membrane structures of bone marrow-derived mast cells. / Zink, T.; Deng, Z.; Chen, H.; Yu, L.; Liu, Fu-Tong; Liu, Gang-yu.

In: Ultramicroscopy, Vol. 109, No. 1, 12.2008, p. 22-31.

Research output: Contribution to journalArticle

Zink, T. ; Deng, Z. ; Chen, H. ; Yu, L. ; Liu, Fu-Tong ; Liu, Gang-yu. / High-resolution three-dimensional imaging of the rich membrane structures of bone marrow-derived mast cells. In: Ultramicroscopy. 2008 ; Vol. 109, No. 1. pp. 22-31.
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