High-resolution optical microscopy imaging of cortical oxygen delivery and consumption

Sava Sakadžic, Emiri T. Mandeville, Louis Gagnon, Joseph J. Musacchia, Mohammad A. Yaseen, A Yucel Meryem A Yucel, Joel Lefebvre, Frédéric Lesage, Anders M. Dale, Katharina Eikermann-Haerter, Cenk Ayata, Vivek J. Srinivasan, Eng H. Lo, Anna Devor, David A. Boas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We applied multimodal microscopic imaging of intravascular partial pressure of O2 (PO2) and blood flow complemented by numerical modeling to reveal the properties of the cortical microvascular oxygenation that allow uninterrupted oxygen delivery to all tissue regions in a very dynamic and energetically demanding brain. Our measurements show that at the baseline level of neuronal activity small arterioles are responsible for a significant oxygen extraction, while the most of the remaining oxygen release is taking place at the level of the first few capillary branches after precapillary arterioles. The majority of capillaries (i.e. high branching order capillaries) release little O2 at rest and our measurements and modeling show that they act as a dynamic O2 reserve that is recruited on demand to ensure adequate tissue oxygenation during increased neuronal activity or decrease in blood flow. These findings have potential to overturn our textbook knowledge of the capillary oxygen delivery, impact our understanding of progression of diseases that affect cerebral microcirculation, and help improving the quantitative interpretation of brain imaging modalities.

Original languageEnglish (US)
Title of host publicationCLEO
Subtitle of host publicationApplications and Technology, CLEO_AT 2014
PublisherOptical Society of America (OSA)
ISBN (Print)9781557529992, 9781557529992
DOIs
StatePublished - Jan 1 2014
EventCLEO: Applications and Technology, CLEO_AT 2014 - San Jose, CA, United States
Duration: Jun 8 2014Jun 13 2014

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Other

OtherCLEO: Applications and Technology, CLEO_AT 2014
CountryUnited States
CitySan Jose, CA
Period6/8/146/13/14

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Sakadžic, S., Mandeville, E. T., Gagnon, L., Musacchia, J. J., Yaseen, M. A., Meryem A Yucel, A. Y., Lefebvre, J., Lesage, F., Dale, A. M., Eikermann-Haerter, K., Ayata, C., Srinivasan, V. J., Lo, E. H., Devor, A., & Boas, D. A. (2014). High-resolution optical microscopy imaging of cortical oxygen delivery and consumption. In CLEO: Applications and Technology, CLEO_AT 2014 (Optics InfoBase Conference Papers). Optical Society of America (OSA). https://doi.org/10.1364/cleo_at.2014.af2b.3