High-resolution optical microscopy imaging of cortical oxygen delivery and consumption

Sava Sakadžić, Emiri T. Mandeville, Louis Gagnon, Joseph J. Musacchia, Mohammad A. Yaseen, Meryem A. Yucel, Joel Lefebvre, Frédéric Lesage, Anders M. Dale, Katharina Eikermann-Haerter, Cenk Ayata, Vivek Srinivasan, Eng H. Lo, Anna Devor, David A. Boas

Research output: Contribution to conferencePaper

Abstract

We applied multimodal microscopic imaging of intravascular partial pressure of O2 (PO2) and blood flow complemented by numerical modeling to reveal the properties of the cortical microvascular oxygenation that allow uninterrupted oxygen delivery to all tissue regions in a very dynamic and energetically demanding brain. Our measurements show that at the baseline level of neuronal activity small arterioles are responsible for a significant oxygen extraction, while the most of the remaining oxygen release is taking place at the level of the first few capillary branches after precapillary arterioles. The majority of capillaries (i.e. high branching order capillaries) release little O2 at rest and our measurements and modeling show that they act as a dynamic O2 reserve that is recruited on demand to ensure adequate tissue oxygenation during increased neuronal activity or decrease in blood flow. These findings have potential to overturn our textbook knowledge of the capillary oxygen delivery, impact our understanding of progression of diseases that affect cerebral microcirculation, and help improving the quantitative interpretation of brain imaging modalities.

Original languageEnglish (US)
StatePublished - Jan 1 2014
Event2014 Conference on Lasers and Electro-Optics, CLEO 2014 - San Jose, United States
Duration: Jun 8 2014Jun 13 2014

Other

Other2014 Conference on Lasers and Electro-Optics, CLEO 2014
CountryUnited States
CitySan Jose
Period6/8/146/13/14

Fingerprint

Optical microscopy
delivery
arterioles
Oxygen
microscopy
Imaging techniques
Oxygenation
oxygenation
high resolution
oxygen
blood flow
brain
Brain
Blood
Tissue
Microcirculation
textbooks
Textbooks
progressions
Partial pressure

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials

Cite this

Sakadžić, S., Mandeville, E. T., Gagnon, L., Musacchia, J. J., Yaseen, M. A., Yucel, M. A., ... Boas, D. A. (2014). High-resolution optical microscopy imaging of cortical oxygen delivery and consumption. Paper presented at 2014 Conference on Lasers and Electro-Optics, CLEO 2014, San Jose, United States.

High-resolution optical microscopy imaging of cortical oxygen delivery and consumption. / Sakadžić, Sava; Mandeville, Emiri T.; Gagnon, Louis; Musacchia, Joseph J.; Yaseen, Mohammad A.; Yucel, Meryem A.; Lefebvre, Joel; Lesage, Frédéric; Dale, Anders M.; Eikermann-Haerter, Katharina; Ayata, Cenk; Srinivasan, Vivek; Lo, Eng H.; Devor, Anna; Boas, David A.

2014. Paper presented at 2014 Conference on Lasers and Electro-Optics, CLEO 2014, San Jose, United States.

Research output: Contribution to conferencePaper

Sakadžić, S, Mandeville, ET, Gagnon, L, Musacchia, JJ, Yaseen, MA, Yucel, MA, Lefebvre, J, Lesage, F, Dale, AM, Eikermann-Haerter, K, Ayata, C, Srinivasan, V, Lo, EH, Devor, A & Boas, DA 2014, 'High-resolution optical microscopy imaging of cortical oxygen delivery and consumption' Paper presented at 2014 Conference on Lasers and Electro-Optics, CLEO 2014, San Jose, United States, 6/8/14 - 6/13/14, .
Sakadžić S, Mandeville ET, Gagnon L, Musacchia JJ, Yaseen MA, Yucel MA et al. High-resolution optical microscopy imaging of cortical oxygen delivery and consumption. 2014. Paper presented at 2014 Conference on Lasers and Electro-Optics, CLEO 2014, San Jose, United States.
Sakadžić, Sava ; Mandeville, Emiri T. ; Gagnon, Louis ; Musacchia, Joseph J. ; Yaseen, Mohammad A. ; Yucel, Meryem A. ; Lefebvre, Joel ; Lesage, Frédéric ; Dale, Anders M. ; Eikermann-Haerter, Katharina ; Ayata, Cenk ; Srinivasan, Vivek ; Lo, Eng H. ; Devor, Anna ; Boas, David A. / High-resolution optical microscopy imaging of cortical oxygen delivery and consumption. Paper presented at 2014 Conference on Lasers and Electro-Optics, CLEO 2014, San Jose, United States.
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AU - Yucel, Meryem A.

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