High-resolution FISH mapping of β-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in cattle standard karyotypes

L. Iannuzzi, D. S. Gallagher, G. P. Di Meo, G. Diamond, Charles L Bevins, J. E. Womack

Research output: Contribution to journalArticle

23 Scopus citations


High-resolution chromosomal fluorescence in situ hybridization (FISH) and R-banding allowed the chromosomal localization of the P-defensin gene cluster (DEFB@) to chromosome band 1p12 in the river buffalo (Bubalus bubalis, 2n = 50) and to band 24q13 in sheep (Ovis aries, 2n = 54), according to standard nomenclatures. The localizations permitted the tentative assignment of the entire bovine syntenic group U25 to these chromosomes. A FISH mapping control on cattle chromosomes confirmed the localization of DEFB@ in the hemoeologous chromosome and chromosome band. Comparisons were made between river buffalo chromosome 1p and the homoeologous cattle chromosome on standard karyotypes; the results are discussed in light of gene assignments, extensive chromosome band conservation among the Bovidae, and inconsistencies in domestic cattle chromosome nomenclatures.

Original languageEnglish (US)
Pages (from-to)10-13
Number of pages4
JournalCytogenetics and Cell Genetics
Issue number1
StatePublished - 1996
Externally publishedYes


ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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