High-resolution FISH mapping of β-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in cattle standard karyotypes

L. Iannuzzi, D. S. Gallagher, G. P. Di Meo, G. Diamond, Charles L Bevins, J. E. Womack

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

High-resolution chromosomal fluorescence in situ hybridization (FISH) and R-banding allowed the chromosomal localization of the P-defensin gene cluster (DEFB@) to chromosome band 1p12 in the river buffalo (Bubalus bubalis, 2n = 50) and to band 24q13 in sheep (Ovis aries, 2n = 54), according to standard nomenclatures. The localizations permitted the tentative assignment of the entire bovine syntenic group U25 to these chromosomes. A FISH mapping control on cattle chromosomes confirmed the localization of DEFB@ in the hemoeologous chromosome and chromosome band. Comparisons were made between river buffalo chromosome 1p and the homoeologous cattle chromosome on standard karyotypes; the results are discussed in light of gene assignments, extensive chromosome band conservation among the Bovidae, and inconsistencies in domestic cattle chromosome nomenclatures.

Original languageEnglish (US)
Pages (from-to)10-13
Number of pages4
JournalCytogenetics and Cell Genetics
Volume75
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

Defensins
Chromosome Mapping
Buffaloes
Fluorescence In Situ Hybridization
Karyotype
Rivers
Sheep
Chromosomes
Domestic Sheep
Multigene Family

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

High-resolution FISH mapping of β-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in cattle standard karyotypes. / Iannuzzi, L.; Gallagher, D. S.; Di Meo, G. P.; Diamond, G.; Bevins, Charles L; Womack, J. E.

In: Cytogenetics and Cell Genetics, Vol. 75, No. 1, 1996, p. 10-13.

Research output: Contribution to journalArticle

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