High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11

Roger V. Lebo, Fredric A Gorin, Robert J. Fletterick, Fa Ten Kao, Mei Chi Cheung, Barry D. Bruce, Yuet Wai Kan

Research output: Contribution to journalArticle

162 Scopus citations

Abstract

A rapid gene-mapping system uses a high-resolution, dual-laser sorter to identify genes from separate human chromosomes prepared with a new stain combination. This system was used to sort 21 unique chromosome types onto nitrocellulose filter papers. Several labeled gene probes hybridized to the sorted chromosomal DNA types predicted by their previous chromosome assignments. The skeletal muscle glycogen phosphorylase gene was then mapped to a portion of chromosome 11 by spot blotting normal and translocated chromosomes.

Original languageEnglish (US)
Pages (from-to)57-59
Number of pages3
JournalScience
Volume225
Issue number4657
StatePublished - 1984

ASJC Scopus subject areas

  • General

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