High-resolution atomic force microscopy studies of the Escherichia coli outer membrane: structural basis for permeability

Nabil A. Amro, Lakshmi P. Kotra, Kapila Wadu-Mesthrige, Alexy Bulychev, Shahriar Mobashery, Gang-yu Liu

Research output: Contribution to journalArticle

315 Citations (Scopus)

Abstract

The structural basis of the outer membrane permeability for the bacterium Escherichia coli is studied by atomic force microscopy (AFM) in conjunction with biochemical treatment and analysis. The surface of the bacterium is visualized with unprecedented detail at 50 and 5 angstrom lateral and vertical resolutions, respectively. The AFM images reveal that the outer membrane of native E. coli exhibits protrusions that correspond to patches of lipopolysaccharide (LPS) containing hundreds to thousands of LPS molecules. The packing of the nearest neighbor patches is tight, and as such the LPS layer provides an effective permeability barrier for the Gram-negative bacteria. Treatment with 50 mM EDTA results in the release of LPS molecules from the boundaries of some patches. Further metal depletion produces many irregularly shaped pits at the outer membrane, which is the consequence of progressive release of LPS molecules and membrane proteins. The EDTA-treated cells were analyzed for metal content and for their reactivities toward lysozyme and antibodies specific for LPS. The experiments collectively indicate that the metal depletion procedure did not remove all the LPS molecules despite a dramatic decrease in the metal content. The remaining LPS molecules are present outside the pits, whereas the bottom of the pits is devoid of these molecules. This new structure for the outer membrane exhibits higher permeability than that for the native cells.

Original languageEnglish (US)
Pages (from-to)2789-2796
Number of pages8
JournalLangmuir
Volume16
Issue number6
DOIs
StatePublished - Mar 21 2000
Externally publishedYes

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Escherichia
Escherichia coli
Lipopolysaccharides
Atomic force microscopy
permeability
atomic force microscopy
membranes
Membranes
Molecules
high resolution
bacteria
molecules
Bacteria
ethylenediaminetetraacetic acids
Metals
Ethylenediaminetetraacetic acid
metals
depletion
Edetic Acid
lysozyme

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

High-resolution atomic force microscopy studies of the Escherichia coli outer membrane : structural basis for permeability. / Amro, Nabil A.; Kotra, Lakshmi P.; Wadu-Mesthrige, Kapila; Bulychev, Alexy; Mobashery, Shahriar; Liu, Gang-yu.

In: Langmuir, Vol. 16, No. 6, 21.03.2000, p. 2789-2796.

Research output: Contribution to journalArticle

Amro, Nabil A. ; Kotra, Lakshmi P. ; Wadu-Mesthrige, Kapila ; Bulychev, Alexy ; Mobashery, Shahriar ; Liu, Gang-yu. / High-resolution atomic force microscopy studies of the Escherichia coli outer membrane : structural basis for permeability. In: Langmuir. 2000 ; Vol. 16, No. 6. pp. 2789-2796.
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