High-resolution ab initio three-dimensional x-ray diffraction microscopy

Henry N. Chapman, Anton Barty, Stefano Marchesini, Aleksandr Noy, Stefan P. Hau-Riege, Congwu Cui, Malcolm R. Howells, Rachel Rosen, Haifeng He, John C H Spence, Uwe Weierstall, Tobias Beetz, Chris Jacobsen, David Shapiro

Research output: Contribution to journalArticle

421 Scopus citations

Abstract

Coherent x-ray diffraction microscopy is a method of imaging nonperiodic isolated objects at resolutions limited, in principle, by only the wavelength and largest scattering angles recorded. We demonstrate x-ray diffraction imaging with high resolution in all three dimensions, as determined by a quantitative analysis of the reconstructed volume images. These images are retrieved from the three-dimensional diffraction data using no a priori knowledge about the shape or composition of the object, which has never before been demonstrated on a nonperiodic object. We also construct two-dimensional images of thick objects with greatly increased depth of focus (without loss of transverse spatial resolution). These methods can be used to image biological and materials science samples at high resolution with x-ray undulator radiation and establishes the techniques to be used in atomic-resolution ultrafast imaging at x-ray free-electron laser sources.

Original languageEnglish (US)
Pages (from-to)1179-1200
Number of pages22
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Volume23
Issue number5
DOIs
StatePublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Computer Vision and Pattern Recognition

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    Chapman, H. N., Barty, A., Marchesini, S., Noy, A., Hau-Riege, S. P., Cui, C., Howells, M. R., Rosen, R., He, H., Spence, J. C. H., Weierstall, U., Beetz, T., Jacobsen, C., & Shapiro, D. (2006). High-resolution ab initio three-dimensional x-ray diffraction microscopy. Journal of the Optical Society of America A: Optics and Image Science, and Vision, 23(5), 1179-1200. https://doi.org/10.1364/JOSAA.23.001179