Abstract
Emerging demands for handling minute liquid samples and reagents have been constantly growing in a wide variety of medical and biological areas. This calls for low-volume and high-precision liquid handling solutions with ease-of-use and portability. In this article, a new digital droplet pipetting method is introduced for the first time, derived from the microfluidic impact printing principle. Configured as a conventional handheld pipette, the prototype device consists of a plug-and-play and disposable microfluidic pipetting chip, driven by a programmable electromagnetic actuator for on-demand dispensing of nanoliter droplets. In particular, the impact-driven microfluidic pipetting chip, in place of the traditional disposable pipette tips, offers both liquid loading and droplet generation. The printing nozzle has been micro-fabricated using a femtosecond laser with a super-hydrophobic structure, in order to minimize the dispensing residues. As a result of the high-precision droplet dispensing principle, the variations of the dispensed volume have been successfully reduced from 49.5% to 0.6% at 0.1 μL, as compared to its commercial counterparts. A proof-of-concept study for concentration dilution and quantitative analysis of cell drug resistance has been carried out by using the digital droplet pipetting system, demonstrating its potential in a broad range of biomedical applications which require both high precision and low-volume processing.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2720-2729 |
| Number of pages | 10 |
| Journal | Lab on a Chip |
| Volume | 18 |
| Issue number | 18 |
| DOIs | |
| State | Published - Jan 1 2018 |
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ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Chemistry(all)
- Biomedical Engineering
Cite this
High-precision digital droplet pipetting enabled by a plug-and-play microfluidic pipetting chip. / Mao, Yuxin; Pan, Yang; Li, Xuan; Li, Baoqing; Chu, Jiaru; Pan, Tingrui.
In: Lab on a Chip, Vol. 18, No. 18, 01.01.2018, p. 2720-2729.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-precision digital droplet pipetting enabled by a plug-and-play microfluidic pipetting chip
AU - Mao, Yuxin
AU - Pan, Yang
AU - Li, Xuan
AU - Li, Baoqing
AU - Chu, Jiaru
AU - Pan, Tingrui
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Emerging demands for handling minute liquid samples and reagents have been constantly growing in a wide variety of medical and biological areas. This calls for low-volume and high-precision liquid handling solutions with ease-of-use and portability. In this article, a new digital droplet pipetting method is introduced for the first time, derived from the microfluidic impact printing principle. Configured as a conventional handheld pipette, the prototype device consists of a plug-and-play and disposable microfluidic pipetting chip, driven by a programmable electromagnetic actuator for on-demand dispensing of nanoliter droplets. In particular, the impact-driven microfluidic pipetting chip, in place of the traditional disposable pipette tips, offers both liquid loading and droplet generation. The printing nozzle has been micro-fabricated using a femtosecond laser with a super-hydrophobic structure, in order to minimize the dispensing residues. As a result of the high-precision droplet dispensing principle, the variations of the dispensed volume have been successfully reduced from 49.5% to 0.6% at 0.1 μL, as compared to its commercial counterparts. A proof-of-concept study for concentration dilution and quantitative analysis of cell drug resistance has been carried out by using the digital droplet pipetting system, demonstrating its potential in a broad range of biomedical applications which require both high precision and low-volume processing.
AB - Emerging demands for handling minute liquid samples and reagents have been constantly growing in a wide variety of medical and biological areas. This calls for low-volume and high-precision liquid handling solutions with ease-of-use and portability. In this article, a new digital droplet pipetting method is introduced for the first time, derived from the microfluidic impact printing principle. Configured as a conventional handheld pipette, the prototype device consists of a plug-and-play and disposable microfluidic pipetting chip, driven by a programmable electromagnetic actuator for on-demand dispensing of nanoliter droplets. In particular, the impact-driven microfluidic pipetting chip, in place of the traditional disposable pipette tips, offers both liquid loading and droplet generation. The printing nozzle has been micro-fabricated using a femtosecond laser with a super-hydrophobic structure, in order to minimize the dispensing residues. As a result of the high-precision droplet dispensing principle, the variations of the dispensed volume have been successfully reduced from 49.5% to 0.6% at 0.1 μL, as compared to its commercial counterparts. A proof-of-concept study for concentration dilution and quantitative analysis of cell drug resistance has been carried out by using the digital droplet pipetting system, demonstrating its potential in a broad range of biomedical applications which require both high precision and low-volume processing.
UR - http://www.scopus.com/inward/record.url?scp=85053637032&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053637032&partnerID=8YFLogxK
U2 - 10.1039/c8lc00505b
DO - 10.1039/c8lc00505b
M3 - Article
C2 - 30014071
AN - SCOPUS:85053637032
VL - 18
SP - 2720
EP - 2729
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 18
ER -