High-performance liquid chromatography for metabolomics

High-efficiency separations utilizing monolithic silica columns

Tohru Ikegami, Hiroshi Kobayashi, Hiroshi Kimura, Vladimir V. Tolstikov, Oliver Fiehn, Nobuo Tanaka

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

5. Summary: Routine use of micro HPLC will need the development of several important constituents; the reproducible preparation of high-performance columns, small-volume pumps and gradient systems, and improvement of an injection system. Subjects to be studied are the development of high-performance monolithic silica columns for a variety of separation modes, multidimensional micro LC systems, and optimization of an interface between LC and MS instruments. Large peak capacities realized by highly efficient micro HPLC systems or multidimensional HPLC will greatly contribute to metabolomics studies when coupled with MS instruments.

Original languageEnglish (US)
Title of host publicationMetabolomics: The Frontier of Systems Biology
PublisherSpringer-Verlag Tokyo
Pages107-126
Number of pages20
ISBN (Print)9784431280552, 4431251219, 9784431251217
DOIs
StatePublished - 2005
Externally publishedYes

Fingerprint

Metabolomics
High performance liquid chromatography
Silicon Dioxide
High Pressure Liquid Chromatography
Pumps
Injections

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ikegami, T., Kobayashi, H., Kimura, H., Tolstikov, V. V., Fiehn, O., & Tanaka, N. (2005). High-performance liquid chromatography for metabolomics: High-efficiency separations utilizing monolithic silica columns. In Metabolomics: The Frontier of Systems Biology (pp. 107-126). Springer-Verlag Tokyo. https://doi.org/10.1007/4-431-28055-3_8

High-performance liquid chromatography for metabolomics : High-efficiency separations utilizing monolithic silica columns. / Ikegami, Tohru; Kobayashi, Hiroshi; Kimura, Hiroshi; Tolstikov, Vladimir V.; Fiehn, Oliver; Tanaka, Nobuo.

Metabolomics: The Frontier of Systems Biology. Springer-Verlag Tokyo, 2005. p. 107-126.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ikegami, T, Kobayashi, H, Kimura, H, Tolstikov, VV, Fiehn, O & Tanaka, N 2005, High-performance liquid chromatography for metabolomics: High-efficiency separations utilizing monolithic silica columns. in Metabolomics: The Frontier of Systems Biology. Springer-Verlag Tokyo, pp. 107-126. https://doi.org/10.1007/4-431-28055-3_8
Ikegami T, Kobayashi H, Kimura H, Tolstikov VV, Fiehn O, Tanaka N. High-performance liquid chromatography for metabolomics: High-efficiency separations utilizing monolithic silica columns. In Metabolomics: The Frontier of Systems Biology. Springer-Verlag Tokyo. 2005. p. 107-126 https://doi.org/10.1007/4-431-28055-3_8
Ikegami, Tohru ; Kobayashi, Hiroshi ; Kimura, Hiroshi ; Tolstikov, Vladimir V. ; Fiehn, Oliver ; Tanaka, Nobuo. / High-performance liquid chromatography for metabolomics : High-efficiency separations utilizing monolithic silica columns. Metabolomics: The Frontier of Systems Biology. Springer-Verlag Tokyo, 2005. pp. 107-126
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