High-performance liquid chromatographic determination of selenocysteine with the fluorescent reagent, N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid

Wayne Chris Hawkes, Mark A. Kutnink

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The method described is based on derivatization of selenocysteine with N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid and responds linearly to selenocysteine spiked into plasma. Recovery is insensitive to inter-individual variation or use of serum versus plasma, but is decreased by hemolysis. The derivative is stable for at least three days. The total imprecision of determinations in plasma was 0.8-2.1% (coefficient of variation) over the range of 6-30 μM selenocysteine, with a detection limit of 0.4 μM (3 × S.D.). There was no significant interference from plasma thiols. This appears to be the first report of the selective reaction of free selenocysteine with a fluorescent reagent. This simple method works well in plasma and serum and may be adaptable to other types of samples.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume576
Issue number2
DOIs
StatePublished - May 8 1992
Externally publishedYes

Fingerprint

Selenocysteine
Plasmas
Liquids
Hemolysis
Serum
Sulfhydryl Compounds
Limit of Detection
1,5-I-AEDANS
Derivatives
Recovery

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

@article{ba599fec4be84a7fab6762afab4eff91,
title = "High-performance liquid chromatographic determination of selenocysteine with the fluorescent reagent, N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid",
abstract = "The method described is based on derivatization of selenocysteine with N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid and responds linearly to selenocysteine spiked into plasma. Recovery is insensitive to inter-individual variation or use of serum versus plasma, but is decreased by hemolysis. The derivative is stable for at least three days. The total imprecision of determinations in plasma was 0.8-2.1{\%} (coefficient of variation) over the range of 6-30 μM selenocysteine, with a detection limit of 0.4 μM (3 × S.D.). There was no significant interference from plasma thiols. This appears to be the first report of the selective reaction of free selenocysteine with a fluorescent reagent. This simple method works well in plasma and serum and may be adaptable to other types of samples.",
author = "Hawkes, {Wayne Chris} and Kutnink, {Mark A.}",
year = "1992",
month = "5",
day = "8",
doi = "10.1016/0378-4347(92)80200-A",
language = "English (US)",
volume = "576",
pages = "263--270",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - High-performance liquid chromatographic determination of selenocysteine with the fluorescent reagent, N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid

AU - Hawkes, Wayne Chris

AU - Kutnink, Mark A.

PY - 1992/5/8

Y1 - 1992/5/8

N2 - The method described is based on derivatization of selenocysteine with N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid and responds linearly to selenocysteine spiked into plasma. Recovery is insensitive to inter-individual variation or use of serum versus plasma, but is decreased by hemolysis. The derivative is stable for at least three days. The total imprecision of determinations in plasma was 0.8-2.1% (coefficient of variation) over the range of 6-30 μM selenocysteine, with a detection limit of 0.4 μM (3 × S.D.). There was no significant interference from plasma thiols. This appears to be the first report of the selective reaction of free selenocysteine with a fluorescent reagent. This simple method works well in plasma and serum and may be adaptable to other types of samples.

AB - The method described is based on derivatization of selenocysteine with N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid and responds linearly to selenocysteine spiked into plasma. Recovery is insensitive to inter-individual variation or use of serum versus plasma, but is decreased by hemolysis. The derivative is stable for at least three days. The total imprecision of determinations in plasma was 0.8-2.1% (coefficient of variation) over the range of 6-30 μM selenocysteine, with a detection limit of 0.4 μM (3 × S.D.). There was no significant interference from plasma thiols. This appears to be the first report of the selective reaction of free selenocysteine with a fluorescent reagent. This simple method works well in plasma and serum and may be adaptable to other types of samples.

UR - http://www.scopus.com/inward/record.url?scp=0026558282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026558282&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(92)80200-A

DO - 10.1016/0378-4347(92)80200-A

M3 - Article

C2 - 1400714

AN - SCOPUS:0026558282

VL - 576

SP - 263

EP - 270

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - 2

ER -