High-performance ion-exchange chromatography of myosin using a DEAE-5PW column

Mark J. Lema, Malcolm G. Pluskal, Paul D. Allen

Research output: Contribution to journalArticle

Abstract

High-performance ion-exchange chromatography of myosin using a DEAE-5PW packing was used to purify myosin from skeletal, cardiac and smooth muscle. This method produces high-speed resolution (30-min analysis) of myosin from contaminating myofibrillar proteins. The column has a high capacity for binding myosin (up to 1 g) and can be used for small-scale preparation of highly purified myosin. Gel analysis in the presence of sodium dodecyl sulfate showed recovery of myosin with very little contamination of other myofibrillar proteins. Myosin was also recovered from small biopsy samples (0.1 g) by a direct extraction technique with recovery of biological ATPase activity.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume491
Issue numberC
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Ion Exchange Chromatography
Myosins
Chromatography
Ion exchange
Skeletal Muscle Myosins
Cardiac Myosins
Smooth Muscle Myosins
Recovery
Biopsy
Sodium Dodecyl Sulfate
Adenosine Triphosphatases
2-diethylaminoethanol
Proteins
Gels
Muscle
Contamination

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

High-performance ion-exchange chromatography of myosin using a DEAE-5PW column. / Lema, Mark J.; Pluskal, Malcolm G.; Allen, Paul D.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 491, No. C, 1989, p. 61-68.

Research output: Contribution to journalArticle

Lema, Mark J. ; Pluskal, Malcolm G. ; Allen, Paul D. / High-performance ion-exchange chromatography of myosin using a DEAE-5PW column. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1989 ; Vol. 491, No. C. pp. 61-68.
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