High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

Igor L. Medintz, Brian M. Paegel, Robert G. Blazej, Charles A. Emrich, Lorenzo Berti, James R. Scherer, Richard A. Mathies

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

This review focuses on some recent advances in realizing microfabricated capillary array electrophoresis (μCAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the high-speed collection of sequencing and genotyping data from radially formatted μCAE devices. The concomitant development of a convenient energy-transfer cassette labeling chemistry allows sensitive multicolor labeling of any DNA genotyping or sequencing analyte. High-performance hereditary haemochromatosis and short tandem repeat genotyping assays are demonstrated on these devices along with rapid mitochondrial DNA sequence polymorphism analysis. Progress in supporting technology such as robotic fluid dispensing and batched data analysis is also presented. The ultimate goal is to develop a parallel analysis platform capable of integrated sample preparation and automated electrophoretic analysis with a throughput 10-100 times that of current technology.

Original languageEnglish (US)
Pages (from-to)3845-3856
Number of pages12
JournalElectrophoresis
Volume22
Issue number18
DOIs
StatePublished - 2001

    Fingerprint

Keywords

  • Capillary array electrophoresis
  • DNA sequencing
  • Energy-transfer fluorescent labels
  • Genetic analysis
  • Microchip
  • Microfabricated genetic analysis devices
  • Review

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Medintz, I. L., Paegel, B. M., Blazej, R. G., Emrich, C. A., Berti, L., Scherer, J. R., & Mathies, R. A. (2001). High-performance genetic analysis using microfabricated capillary array electrophoresis microplates. Electrophoresis, 22(18), 3845-3856. https://doi.org/10.1002/1522-2683(200110)22:18<3845::AID-ELPS3845>3.0.CO;2-0