High-level synthesis and secretion of α-amylase from rice callus

Carl R. Simmons, Raymond L. Rodriguez

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

Rice seed callus was found to synthesize and secrete high levels of α-amylase for up to 17 months after initiation. Approximately 43% of the total secreted protein is α-amylase. This level of expression was not increased by exogenous gibberellic acid, nor was expression reduced by the gibberellin biosynthesis inhibitor paclobutrazol. Alpha-amylase expression, however, was reduced 41-fold in seed callus derived from Tan-ginbozu, a mutant lacking gibberellins, suggesting that gibberellins are involved in the regulation of α-amylase biosynthesis in the callus. This low expression in Tan-ginbozu callus, however, was not increased by exogenous gibberellic acid. Surprisingly, shoot-derived callus also secreted α-amylase at levels similar to seed-derived callus, even though shoot tissue normally produces only low levels of intracellular α-amylase.

Original languageEnglish (US)
Title of host publicationACS Symposium Series
PublisherPubl by ACS
Pages202-214
Number of pages13
Edition389
Publication statusPublished - 1989
EventThird Chemical Congress of North America (195th National Meeting of the American Chemical Society) - Biocatalysis in Agricultural Biotechnology - Toronto, Ont, Can
Duration: Jun 5 1988Jun 11 1988

Other

OtherThird Chemical Congress of North America (195th National Meeting of the American Chemical Society) - Biocatalysis in Agricultural Biotechnology
CityToronto, Ont, Can
Period6/5/886/11/88

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ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Simmons, C. R., & Rodriguez, R. L. (1989). High-level synthesis and secretion of α-amylase from rice callus. In ACS Symposium Series (389 ed., pp. 202-214). Publ by ACS.