High-level expression of human αs1-casein in Escherichia coli

Y. K. Kim, B. H. Chung, S. Yoon, K. K. Lee, B. Lönnerdal, D. Y. Yu

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Human αs1-casein was expressed efficiently in Escherichia coli. The overproduced recombinant human αs1-casein was about 25% of the total cell protein. Two different vectors were constructed to express Met-αs1-casein and Met-αs1-casein with a His-affinity tag at the C-terminus. Recombinant Met-αs1-casein with a His-affinity tag was purified to homogeneity using Ni-nitrilotriacetic acid resin. n-terminal sequence of the first 10 amino acid residues of this purified protein was identical to that of mature human αs1-casein with an extra methionine residue at the N-terminus.

Original languageEnglish (US)
Pages (from-to)675-678
Number of pages4
JournalBiotechnology Techniques
Volume11
Issue number9
StatePublished - 1997

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

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