High-level expression of human αs1-casein in Escherichia coli

Y. K. Kim, B. H. Chung, S. Yoon, K. K. Lee, B. Lönnerdal, D. Y. Yu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Human αs1-casein was expressed efficiently in Escherichia coli. The overproduced recombinant human αs1-casein was about 25% of the total cell protein. Two different vectors were constructed to express Met-αs1-casein and Met-αs1-casein with a His-affinity tag at the C-terminus. Recombinant Met-αs1-casein with a His-affinity tag was purified to homogeneity using Ni-nitrilotriacetic acid resin. n-terminal sequence of the first 10 amino acid residues of this purified protein was identical to that of mature human αs1-casein with an extra methionine residue at the N-terminus.

Original languageEnglish (US)
Pages (from-to)675-678
Number of pages4
JournalBiotechnology Techniques
Volume11
Issue number9
StatePublished - 1997

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Casein
Caseins
Escherichia coli
Nitrilotriacetic Acid
Proteins
Methionine
Amino acids
Resins
Amino Acids
Acids

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Kim, Y. K., Chung, B. H., Yoon, S., Lee, K. K., Lönnerdal, B., & Yu, D. Y. (1997). High-level expression of human αs1-casein in Escherichia coli. Biotechnology Techniques, 11(9), 675-678.

High-level expression of human αs1-casein in Escherichia coli. / Kim, Y. K.; Chung, B. H.; Yoon, S.; Lee, K. K.; Lönnerdal, B.; Yu, D. Y.

In: Biotechnology Techniques, Vol. 11, No. 9, 1997, p. 675-678.

Research output: Contribution to journalArticle

Kim, YK, Chung, BH, Yoon, S, Lee, KK, Lönnerdal, B & Yu, DY 1997, 'High-level expression of human αs1-casein in Escherichia coli', Biotechnology Techniques, vol. 11, no. 9, pp. 675-678.
Kim YK, Chung BH, Yoon S, Lee KK, Lönnerdal B, Yu DY. High-level expression of human αs1-casein in Escherichia coli. Biotechnology Techniques. 1997;11(9):675-678.
Kim, Y. K. ; Chung, B. H. ; Yoon, S. ; Lee, K. K. ; Lönnerdal, B. ; Yu, D. Y. / High-level expression of human αs1-casein in Escherichia coli. In: Biotechnology Techniques. 1997 ; Vol. 11, No. 9. pp. 675-678.
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