High incidence of glomerulonephritis associated with inclusion body hepatitis in broiler chickens: Routine histopathology and histomorphometric studies

Floyd D. Wilson, Robert W. Wills, Carlos G Senties-cue, William R. Maslin, Philip A. Stayer, Danny L. Magee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

During the routine histologic evaluation of an outbreak of inclusion body hepatitis (IBH) in Mississippi broilers, a high incidence of renal enlargement and glomerulonephropathy was observed in the birds presenting classic hepatic pathology. Characteristic intranuclear adenoviral inclusion bodies were demonstrated in the livers of these birds, and fowl adenovirus was identified by viral isolation and by PCR. The glomerular lesions were consistent with proliferative or membranoproliferative forms of glomerulonephritis. Histomorphometric evaluations were performed to generate a more quantitative analysis of altered glomerular size and cellularity, to detect statistically significant borderline changes, and to get a clearer insight into the incidence of the glomerular alterations. Marked increases in both the average glomerular size (area) and the total glomerular cellularity were observed for the affected glomeruli relative to normal controls. The average glomerular area values for normal glomeruli in the peripheral subcapsular cortical and central cortical kidney regions were 1791 μm2 and 5302 μm2, respectively. In contrast, glomerular measurements for kidneys exhibiting glomerulonephritis by routine histopathology, had average values for the two regions of 4429 μm2 and 11,063 μm2. The average glomerular cell counts for the two regions in controls were 44 and 107 cells/glomeruli, while averages for birds with glomerulonephritis were 85 and 193 cells/glomeruli. The proportion of IBH-associated glomeruli greater than two standard deviations above the mean glomerular size of the normal controls was 52 for the central region and 62 for the peripheral region.

Original languageEnglish (US)
Pages (from-to)975-980
Number of pages6
JournalAvian Diseases
Volume54
Issue number3
DOIs
StatePublished - Sep 2010

Fingerprint

glomerulonephritis
Inclusion Bodies
inclusion bodies
hepatitis
Glomerulonephritis
histopathology
Hepatitis
Birds
Chickens
broiler chickens
kidneys
Kidney
incidence
birds
Incidence
nuclear inclusions
Membranoproliferative Glomerulonephritis
Mississippi
Intranuclear Inclusion Bodies
liver

Keywords

  • glomerulonephritis
  • histomorphometrics
  • inclusion body nephritis
  • poultry

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Cite this

High incidence of glomerulonephritis associated with inclusion body hepatitis in broiler chickens : Routine histopathology and histomorphometric studies. / Wilson, Floyd D.; Wills, Robert W.; Senties-cue, Carlos G; Maslin, William R.; Stayer, Philip A.; Magee, Danny L.

In: Avian Diseases, Vol. 54, No. 3, 09.2010, p. 975-980.

Research output: Contribution to journalArticle

Wilson, Floyd D. ; Wills, Robert W. ; Senties-cue, Carlos G ; Maslin, William R. ; Stayer, Philip A. ; Magee, Danny L. / High incidence of glomerulonephritis associated with inclusion body hepatitis in broiler chickens : Routine histopathology and histomorphometric studies. In: Avian Diseases. 2010 ; Vol. 54, No. 3. pp. 975-980.
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