High frequency of the PNPLA3 rs738409 [G] single-nucleotide polymorphism in Hmong individuals as a potential basis for a predisposition to chronic liver disease

Clifford G Tepper, Julie H.T. Dang, Susan L. Stewart, Dao M. Fang, Kimberly A. Wong, Stephenie Y. Liu, Ryan R. Davis, Doan Y. Dao, Jeffrey P. Gregg, Natalie J. Török, Moon S. Chen

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

BACKGROUND: An exploratory study was performed to determine the prevalence of the patatin-like phospholipase domain-containing protein 3 (PNPLA3) rs78409 [G] allele among the Hmong as a risk factor for nonalcoholic fatty liver disease (NAFLD). NAFLD/nonalcoholic steatohepatitis is the world's most common chronic liver disease and is expected to replace viral hepatitis as the leading cause of cirrhosis and potential precursor to hepatocellular carcinoma (HCC). Of all populations in California, the Hmong experience the highest risk of death from HCC and the highest prevalence of metabolic syndrome risk factors among Asians that predispose them to NAFLD. Here a genetic explanation was sought for the high rates of chronic liver disease among the Hmong. The literature pointed to the PNPLA3 rs738409 [G] allele as a potential genetic culprit. METHODS: Cell-free DNA was isolated from 26 serum samples previously collected in community settings. Quantitative polymerase chain reaction–based single-nucleotide polymorphism (SNP) genotyping was performed with a validated TaqMan SNP genotyping assay, and results were analyzed with TaqMan Genotyper software. RESULTS: The PNPLA3 rs738409 [C>G] variant occurred at a frequency of 0.46 (12 of 26; 95% confidence interval, 0.27-0.67). This carrier rate would rank the Hmong as the third highest population in the 1000 Genomes Project. CONCLUSIONS: Although this small sample size limits the generalizability, the high frequency rates of this allele along with the presence of metabolic syndrome risk factors warrant further studies into the etiology of NAFLD among the Hmong. Cancer 2018;124:1583-9.

Original languageEnglish (US)
Pages (from-to)1583-1589
Number of pages7
JournalCancer
Volume124
DOIs
StatePublished - Apr 1 2018

Keywords

  • carrier rate
  • hepatocellular carcinoma
  • Hmong
  • nonalcoholic steatohepatitis (NASH)
  • patatin-like phospholipase domain-containing protein 3 (PNPLA3) rs738409

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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