High fat feeding increases brown fat GDP binding in lean but not obese Zucker rats

T. W L Gong, J. S. Stern, Barbara A Horwitz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effects of high fat feeding on brown fat thermogenesis in rodents are controversial. In this study, we examined the effects of 8 d of high fat feeding on brown fat mitochondrial GDP binding (an in vitro index of thermogenic activity) in lean (Fa/?) and obese (fa/fa) Zucker rats. Six-week-old female rats were fed a defined low fat control diet (9.5% of energy from fat) only during the dark cycle (1200-2400 h), and food intake was measured daily at 1200, 1600, 2300, and 2400 h for 7 d (the control period). For the next 8 d, half of the lean and obese rats were fed a high fat diet (65% of energy from fat), and the others remained on the low fat control diet. Each rat was fed the amount of energy equivalent to its average energy intake during the 7-d control period. Rats were killed at 0800-1000 h. In the lean rats, high fat feeding increased GDP binding. This increased binding in the lean rats appeared to reflect levels of dietary fat and carbohydrate and was independent of caloric intake. The blunted GDP binding of the obese rats failed to respond to dietary changes.

Original languageEnglish (US)
Pages (from-to)786-792
Number of pages7
JournalJournal of Nutrition
Volume120
Issue number7
StatePublished - 1990

Fingerprint

Zucker Rats
Brown Adipose Tissue
brown adipose tissue
Fats
rats
lipids
Fat-Restricted Diet
Energy Intake
energy intake
energy
Dietary Carbohydrates
Thermogenesis
Dietary Fats
High Fat Diet
dietary carbohydrate
high fat diet
heat production
Rodentia
diet
dietary fat

Keywords

  • Brown fat
  • GDP binding
  • Mitochondria
  • Thermogenesis
  • Zucker rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Gong, T. W. L., Stern, J. S., & Horwitz, B. A. (1990). High fat feeding increases brown fat GDP binding in lean but not obese Zucker rats. Journal of Nutrition, 120(7), 786-792.

High fat feeding increases brown fat GDP binding in lean but not obese Zucker rats. / Gong, T. W L; Stern, J. S.; Horwitz, Barbara A.

In: Journal of Nutrition, Vol. 120, No. 7, 1990, p. 786-792.

Research output: Contribution to journalArticle

Gong, TWL, Stern, JS & Horwitz, BA 1990, 'High fat feeding increases brown fat GDP binding in lean but not obese Zucker rats', Journal of Nutrition, vol. 120, no. 7, pp. 786-792.
Gong, T. W L ; Stern, J. S. ; Horwitz, Barbara A. / High fat feeding increases brown fat GDP binding in lean but not obese Zucker rats. In: Journal of Nutrition. 1990 ; Vol. 120, No. 7. pp. 786-792.
@article{ef46b6233e4a463eb7bac5af10533ff3,
title = "High fat feeding increases brown fat GDP binding in lean but not obese Zucker rats",
abstract = "The effects of high fat feeding on brown fat thermogenesis in rodents are controversial. In this study, we examined the effects of 8 d of high fat feeding on brown fat mitochondrial GDP binding (an in vitro index of thermogenic activity) in lean (Fa/?) and obese (fa/fa) Zucker rats. Six-week-old female rats were fed a defined low fat control diet (9.5{\%} of energy from fat) only during the dark cycle (1200-2400 h), and food intake was measured daily at 1200, 1600, 2300, and 2400 h for 7 d (the control period). For the next 8 d, half of the lean and obese rats were fed a high fat diet (65{\%} of energy from fat), and the others remained on the low fat control diet. Each rat was fed the amount of energy equivalent to its average energy intake during the 7-d control period. Rats were killed at 0800-1000 h. In the lean rats, high fat feeding increased GDP binding. This increased binding in the lean rats appeared to reflect levels of dietary fat and carbohydrate and was independent of caloric intake. The blunted GDP binding of the obese rats failed to respond to dietary changes.",
keywords = "Brown fat, GDP binding, Mitochondria, Thermogenesis, Zucker rats",
author = "Gong, {T. W L} and Stern, {J. S.} and Horwitz, {Barbara A}",
year = "1990",
language = "English (US)",
volume = "120",
pages = "786--792",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "7",

}

TY - JOUR

T1 - High fat feeding increases brown fat GDP binding in lean but not obese Zucker rats

AU - Gong, T. W L

AU - Stern, J. S.

AU - Horwitz, Barbara A

PY - 1990

Y1 - 1990

N2 - The effects of high fat feeding on brown fat thermogenesis in rodents are controversial. In this study, we examined the effects of 8 d of high fat feeding on brown fat mitochondrial GDP binding (an in vitro index of thermogenic activity) in lean (Fa/?) and obese (fa/fa) Zucker rats. Six-week-old female rats were fed a defined low fat control diet (9.5% of energy from fat) only during the dark cycle (1200-2400 h), and food intake was measured daily at 1200, 1600, 2300, and 2400 h for 7 d (the control period). For the next 8 d, half of the lean and obese rats were fed a high fat diet (65% of energy from fat), and the others remained on the low fat control diet. Each rat was fed the amount of energy equivalent to its average energy intake during the 7-d control period. Rats were killed at 0800-1000 h. In the lean rats, high fat feeding increased GDP binding. This increased binding in the lean rats appeared to reflect levels of dietary fat and carbohydrate and was independent of caloric intake. The blunted GDP binding of the obese rats failed to respond to dietary changes.

AB - The effects of high fat feeding on brown fat thermogenesis in rodents are controversial. In this study, we examined the effects of 8 d of high fat feeding on brown fat mitochondrial GDP binding (an in vitro index of thermogenic activity) in lean (Fa/?) and obese (fa/fa) Zucker rats. Six-week-old female rats were fed a defined low fat control diet (9.5% of energy from fat) only during the dark cycle (1200-2400 h), and food intake was measured daily at 1200, 1600, 2300, and 2400 h for 7 d (the control period). For the next 8 d, half of the lean and obese rats were fed a high fat diet (65% of energy from fat), and the others remained on the low fat control diet. Each rat was fed the amount of energy equivalent to its average energy intake during the 7-d control period. Rats were killed at 0800-1000 h. In the lean rats, high fat feeding increased GDP binding. This increased binding in the lean rats appeared to reflect levels of dietary fat and carbohydrate and was independent of caloric intake. The blunted GDP binding of the obese rats failed to respond to dietary changes.

KW - Brown fat

KW - GDP binding

KW - Mitochondria

KW - Thermogenesis

KW - Zucker rats

UR - http://www.scopus.com/inward/record.url?scp=0025359044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025359044&partnerID=8YFLogxK

M3 - Article

C2 - 2195145

AN - SCOPUS:0025359044

VL - 120

SP - 786

EP - 792

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 7

ER -