High dietary protein and taurine increase cysteine desulfhydration in kittens

Research output: Contribution to journalArticle

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Abstract

The objective of this study was to determine the effect of dietary protein and taurine on cysteine desulfhydration in various kitten tissues. Cysteine desulfhydration was assessed in liver, kidney, skeletal muscle, heart, spleen, brain and jejunum of kittens fed one of the following diets for 5 wk: 20% protein, 0% taurine diet (LPOT); 20% protein, 0.15% taurine diet (LPNT); 60% protein, 0% taurine diet (HPOT); and 60% protein, 0.15% taurine diet (HPNT). Cats fed LPOT and HPOT had been fed a taurine-free diet for 10 wk before the 5-wk experiment. The activity of cysteine desulfhydration was determined by measuring the production of H2 35S from 35S-cysteine in the presence and absence of α-ketoglutarate (αKG) in the incubation medium. Liver and kidney had the highest total activities among the tissues tested (P < 0.01). Total hepatic desulfhydration activities [μmol H2S/(min · kg body wt)] in cats fed LPOT, LPNT, HPOT and HPNT were (mean ± SEM) 117 ± 6, 135 ± 10, 137 ± 10 and 190 ± 9, respectively. Dietary taurine had a significant effect on activity when expressed per gram liver (P < 0.01), per gram protein (P < 0.05) and per kilogram body weight (P < 0.001). Dietary protein had a significant effect (P < 0.001) only when activity was expressed relative to body weight because of the significant effect of protein on relative liver weight. The direct pathway via cysteine desulfhydrase appears to be the major route of cysteine desulfhydration in kitten liver because the values obtained in the absence of αKG were 81-88% of those obtained in the presence of αKG.

Original languageEnglish (US)
Pages (from-to)2225-2230
Number of pages6
JournalJournal of Nutrition
Volume129
Issue number12
StatePublished - Dec 8 1999

Fingerprint

Dietary Proteins
kittens
Taurine
taurine
dietary protein
Cysteine
cysteine
Diet
liver
Liver
diet
Proteins
proteins
Cats
Cystathionine gamma-Lyase
Body Weight
kidneys
cats
Kidney
hydrogen production

Keywords

  • Cysteine desulfhydrase
  • Cysteine desutfhydration
  • Dietary protein
  • Dietary taurine
  • Kittens

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

High dietary protein and taurine increase cysteine desulfhydration in kittens. / Park, Taesun; Rogers, Quinton; Morris, James.

In: Journal of Nutrition, Vol. 129, No. 12, 08.12.1999, p. 2225-2230.

Research output: Contribution to journalArticle

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