High-affinity anti-ganglioside IgG antibodies raised in complex ganglioside knockout mice: Reexamination of GD1a immunolocalization

Michael P T Lunn, L'Aurelle A. Johnson, Susan E. Fromholt, Saki Itonori, Jian Huang, Alka A. Vyas, James Hildreth, John W. Griffin, Ronald L. Schnaar, Kazim A. Sheikh

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

Gangliosides, sialic acid-bearing glycosphingolipids, are highly enriched in the vertebrate nervous system. Anti-ganglioside antibodies are associated with various human neuropathies, although the pathogenicity of these antibodies remains unproven. Testing the pathogenic role of anti- ganglioside antibodies will be facilitated by developing high-affinity IgG- class complement-fixing monoclonal antibodies against major brain gangliosides, a goal that has been difficult to achieve. In this study, mice lacking complex gangliosides were used as immune-naive hosts to raise anti- ganglioside antibodies. Wild-type mice and knockout mice with a disrupted gene for GM2/GD2 synthase (UDP-N-acetyl-D-galactosamine:GM3/GD3 N-acetyl-D- galactosaminyltransferase) were immunized with GD1a conjugated to keyhole limpet hemocyanin. The knockout mice produced a vigorous anti-GD1a IgG response, whereas wild-type littermates failed to do so. Fusion of spleen cells from an immunized knockout mouse with myeloma cells yielded numerous IgG anti-GD1a antibody-producing colonies. Ganglioside binding studies revealed two specificity classes; one colony representing each class was cloned and characterized. High-affinity monoclonal antibody was produced by each hybridoma: an IgG1 that bound nearly exclusively to GD1a and an IgG2b that bound GD1a, GT1b, and GT1aα. Both antibodies readily detected gangliosides via ELISA, TLC immune overlay, immunohistochemistry, and immunocytochemistry. In contrast to prior reports using anti-GD1a and anti- GT1b IgM class monoclonal antibodies, the new antibodies bound avidly to granule neurons in brain tissue sections and cell cultures. Mice lacking complex gangliosides are improved hosts for raising high-affinity, high-titer anti-ganglioside IgG antibodies for probing for the distribution and physiology of gangliosides and the pathophysiology of anti-ganglioside antibodies.

Original languageEnglish (US)
Pages (from-to)404-412
Number of pages9
JournalJournal of Neurochemistry
Volume75
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

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Keywords

  • Gangliosides
  • GD1a
  • GM2/GD2 synthase
  • GT1b
  • Knockout mice
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Lunn, M. P. T., Johnson, LA. A., Fromholt, S. E., Itonori, S., Huang, J., Vyas, A. A., Hildreth, J., Griffin, J. W., Schnaar, R. L., & Sheikh, K. A. (2000). High-affinity anti-ganglioside IgG antibodies raised in complex ganglioside knockout mice: Reexamination of GD1a immunolocalization. Journal of Neurochemistry, 75(1), 404-412. https://doi.org/10.1046/j.1471-4159.2000.0750404.x