HIF1A P582S gene association with endurance training responses in young women

J. S. McPhee, J. Perez-Schindler, H. Degens, D. Tomlinson, P. Hennis, Keith Baar, A. G. Williams

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Sequence variations in the gene encoding the hypoxia-inducible factor-1alpha, HIF1A, have been associated with physiologic function and could be associated with exercise responses. In the HIF1A P582S gene polymorphism (C1772T; rs 11549465 C/T), a single nucleotide transition from C → T alters the codon sequence from the usual amino acid; proline (C-allele), to serine (T-allele). This polymorphism was examined for association with endurance training responses in 58 untrained young women who completed a 6-week laboratory-based endurance training programme. Participant groups were defined as CC homozygotes versus carriers of a T-allele (CC vs. CT genotypes). Adaptations were examined at the systemiclevel, by measuring VO 2max and the molecular-level by measuring enzymes determined from vastus lateralis (n = 20): 3-hydroacyl-CoA-dehydrogenase (HAD), which regulates mitochondrial fatty acid oxidation; cytochrome C oxidase (COX-1), a marker of mitochondrial density; and phosphofructokinase (PFK), a marker of glycolytic capacity. CT genotypes showed 45% higher training-induced gains in VO 2max compared with CC genotypes (P<0.05). At the molecular level, CT increased the ratios PFK/HAD and PFK/COX-1 (47 and 3%, respectively), while in the CC genotypes these ratios were decreased (-26 and -54%, respectively). In conclusion, the T-allele of HIF1A P582S was associated with greater gains in VO 2max following endurance training in young women. In a sub-group we also provide preliminary evidence of differential muscle metabolic adaptations between genotypes.

Original languageEnglish (US)
Pages (from-to)2339-2347
Number of pages9
JournalEuropean Journal of Applied Physiology
Volume111
Issue number9
DOIs
StatePublished - Sep 2011

Keywords

  • Exercise
  • Hypoxia
  • Oxygen uptake
  • Polymorphism
  • Skeletal muscle

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Orthopedics and Sports Medicine
  • Physiology (medical)

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    McPhee, J. S., Perez-Schindler, J., Degens, H., Tomlinson, D., Hennis, P., Baar, K., & Williams, A. G. (2011). HIF1A P582S gene association with endurance training responses in young women. European Journal of Applied Physiology, 111(9), 2339-2347. https://doi.org/10.1007/s00421-011-1869-4