Hex1: A new human Rad2 nuclease family member with homology to yeast exonuclease 1

David M. Wilson, James P. Carney, Matthew A Coleman, Aaron W. Adamson, Mari Christensen, Jane E. Lamerdin

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Nucleolytic processing of chromosomal DNA is required in operations such as DNA repair, recombination and replication. We have identified a human gene, named HEX1 for human exonuclease 1, by searching the EST database for cDNAs that encode a homolog to the Saccharomyces cerevisiae EXO1 gene product. Based on its homology to this and other DNA repair proteins of the Rad2 family, most notably Schizosaccharomyces pombe exonuclease 1 (Exo1), Hex1 presumably functions as a nuclease in aspects of recombination or mismatch repair. Similar to the yeast proteins, recombinant Hex1 exhibits a 5'→3' exonuclease activity. Northern blot analysis revealed that HEX1 expression is highest in fetal liver and adult bone marrow, suggesting that the encoded protein may operate prominently in processes specific to hemopoietic stem cell development. HEX1 gene equivalents were found in all vertebrates examined. The human gene includes 14 exons and 13 introns that span ~42 kb of genomic DNA and maps to the chromosomal position 1q42-43, a region lost in some cases of acute leukemia and in several solid tumors.

Original languageEnglish (US)
Pages (from-to)3762-3768
Number of pages7
JournalNucleic Acids Research
Volume26
Issue number16
DOIs
StatePublished - Aug 15 1998
Externally publishedYes

Fingerprint

Yeasts
DNA Repair
Genes
Recombinational DNA Repair
Exonucleases
DNA Mismatch Repair
Fungal Proteins
Schizosaccharomyces
DNA
Expressed Sequence Tags
DNA Replication
Northern Blotting
Introns
Genetic Recombination
Saccharomyces cerevisiae
Vertebrates
Exons
Leukemia
Proteins
Stem Cells

ASJC Scopus subject areas

  • Genetics

Cite this

Wilson, D. M., Carney, J. P., Coleman, M. A., Adamson, A. W., Christensen, M., & Lamerdin, J. E. (1998). Hex1: A new human Rad2 nuclease family member with homology to yeast exonuclease 1. Nucleic Acids Research, 26(16), 3762-3768. https://doi.org/10.1093/nar/26.16.3762

Hex1 : A new human Rad2 nuclease family member with homology to yeast exonuclease 1. / Wilson, David M.; Carney, James P.; Coleman, Matthew A; Adamson, Aaron W.; Christensen, Mari; Lamerdin, Jane E.

In: Nucleic Acids Research, Vol. 26, No. 16, 15.08.1998, p. 3762-3768.

Research output: Contribution to journalArticle

Wilson, DM, Carney, JP, Coleman, MA, Adamson, AW, Christensen, M & Lamerdin, JE 1998, 'Hex1: A new human Rad2 nuclease family member with homology to yeast exonuclease 1', Nucleic Acids Research, vol. 26, no. 16, pp. 3762-3768. https://doi.org/10.1093/nar/26.16.3762
Wilson, David M. ; Carney, James P. ; Coleman, Matthew A ; Adamson, Aaron W. ; Christensen, Mari ; Lamerdin, Jane E. / Hex1 : A new human Rad2 nuclease family member with homology to yeast exonuclease 1. In: Nucleic Acids Research. 1998 ; Vol. 26, No. 16. pp. 3762-3768.
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