Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos

Robert L. Morris, Jonathan M. Scholey

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Heterotrimeric kinesin-II is a plus end-directed microtubule (MT) motor protein consisting of distinct heterodimerized motor subunits associated with an accessory subunit. To probe the intracellular transport functions of kinesin-II, we microinjected fertilized sea urchin eggs with an anti-kinesin- II monoclonal antibody, and we observed a dramatic inhibition of ciliogenesis at the blastula stage characterized by the assembly of short, paralyzed, 9+0 ciliary axonemes that lack central pair MTs. Control embryos show no such defect and form swimming blastulae with normal, motile, 9+2 cilia that contain kinesin-II as detected by Western blotting. Injection of anti- kinesin-II into one blastomere of a two-cell embryo leads to the development of chimeric blastulae covered on one side with short, paralyzed cilia, and on the other with normal, beating cilia. We observed a unimodal length distribution of short cilia on anti-kinesin-II-injected embryos corresponding to the first mode of the trimodal distribution of ciliary lengths observed for control embryos. This short mode may represent a default ciliary assembly intermediate. We hypothesize that kinesin-II functions during ciliogenesis to deliver ciliary components that are required for elongation of the assembly inter-mediate and for formation of stable central pair MTs. Thus, kinesin-II plays a critical role in embryonic development by supporting the maturation of nascent cilia to generate long motile organelles capable of producing the propulsive forces required for swimming and feeding.

Original languageEnglish (US)
Pages (from-to)1009-1022
Number of pages14
JournalJournal of Cell Biology
Volume138
Issue number5
DOIs
StatePublished - Sep 8 1997

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Axoneme
Sea Urchins
Embryonic Structures
Cilia
Blastula
Microtubule Proteins
Blastomeres
kinesin-II
Organelles
Eggs
Embryonic Development
Western Blotting
Monoclonal Antibodies
Injections

ASJC Scopus subject areas

  • Cell Biology

Cite this

Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos. / Morris, Robert L.; Scholey, Jonathan M.

In: Journal of Cell Biology, Vol. 138, No. 5, 08.09.1997, p. 1009-1022.

Research output: Contribution to journalArticle

Morris, Robert L. ; Scholey, Jonathan M. / Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos. In: Journal of Cell Biology. 1997 ; Vol. 138, No. 5. pp. 1009-1022.
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