Heterologous in vitro transcription from two archaebacterial promoters

Uwe Hüdepohl, Felix Gropp, Mary C Horne, Wolfram Zillig

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A cell-free extract of Sulfolobus shibatae is able to specifically initiate transcription in vitro at the promoter of the plasmid-encoded gene for the major gas vesicle protein of Halobacterium halobium and at the promoter for the transcript T4 of the temperate H. halobium phage ΦH. The corresponding promoter from the virulent phage mutant ΦHL1 yields enhanced transcription in the heterologous system, in agreement with strongly increased in vivo expression.

Original languageEnglish (US)
Pages (from-to)257-259
Number of pages3
JournalFEBS Letters
Volume285
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

Keywords

  • Cell-free extract
  • Evolution
  • Gas vacuole gene
  • Phage ΦH
  • Promoter
  • Transcription initiation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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