Heterogeneous immunoreactivity of axonal spheroids in focal symmetrical encephalomalacia produced by Clostridium perfringens type D epsilon toxin in sheep

Jim Manavis, Peter Blumbergs, Ian Jerrett, Daren Hanshaw, Francisco Uzal, John Finnie

Research output: Contribution to journalArticlepeer-review

Abstract

Since axonal injury (AI) is an important component of many veterinary neurologic disorders, we assessed the relative ability of a panel of antibodies (amyloid precursor protein, 3 subunits of neurofilament protein, protein gene product 9.5, ubiquitin, and synaptophysin) to detect axonal swellings or spheroids. Abundant axonal spheroids found in necrotic internal capsule foci produced in 4 sheep by chronic Clostridium perfringens type D epsilon neurotoxicity provided a model system in which to evaluate this important diagnostic tool. There was heterogeneous labeling of subsets of spheroids by the respective antibodies, suggesting that, in order to detect the complete spectrum of AI in diagnostic cases, a range of antibodies should be used, not only when spheroids are plentiful but also when they are few in number or incompletely developed. The application of insufficient markers in the latter cases can potentially lead to the contribution of AI to lesion pathogenesis being underappreciated.

Original languageEnglish (US)
Pages (from-to)328-332
Number of pages5
JournalVeterinary pathology
Volume59
Issue number2
DOIs
StatePublished - Mar 2022

Keywords

  • axonal spheroids
  • brain
  • clostridial epsilon toxin
  • immunohistochemistry
  • sheep

ASJC Scopus subject areas

  • veterinary(all)

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