Heterogeneity of rat hepatic Ah receptor

identification of two receptor forms which differ in their biochemical properties.

M. S. Denison

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In cytosol, the rat hepatic Ah receptor (AhR) appears to exist in two distinct forms (AhR alpha, AhR beta) in similar concentration. The binding of ligand (2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)) to AhR alpha requires the receptor be in its oligomeric 8-10 to S conformation (bound to other protein subunits), while ligand binding to AhR beta can occur with the dissociated 5-6 S form. Occupancy of AhR alpha by ligand (TCDD) protects it from salt-dependent inactivation; AhR beta is not inactivated by high salt conditions. The addition of molybdate to cytosol during tissue homogenization stabilized AhR alpha against salt-dependent inactivation and subunit dissociation but did not prevent dissociation of AhR beta by high salt. Although the presence of molybdate appears to stabilize AhR alpha in its oligomeric 8-10 S, it had no significant effect on the overall amount of TCDD:AhR complex which bound to its specific DNA recognition site, the dioxin responsive element (DRE). These results suggest that AhR alpha, unlike AhR beta, is either unable to transform or bind to the DRE with high affinity.

Original languageEnglish (US)
Pages (from-to)249-256
Number of pages8
JournalJournal of Biochemical Toxicology
Volume7
Issue number4
StatePublished - Dec 1992
Externally publishedYes

Fingerprint

Rats
Salts
Dioxins
Liver
Ligands
Cytosol
Aryl Hydrocarbon Receptors
Protein Subunits
Conformations
Tissue
DNA
1,4-dioxin
Polychlorinated Dibenzodioxins
molybdate

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Heterogeneity of rat hepatic Ah receptor : identification of two receptor forms which differ in their biochemical properties. / Denison, M. S.

In: Journal of Biochemical Toxicology, Vol. 7, No. 4, 12.1992, p. 249-256.

Research output: Contribution to journalArticle

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