Heterogeneity of dendritic cells in the mouse liver: Identification and characterization of four distinct populations

Zhe Xiong Lian, Tomoyuki Okada, Xiaosong He, Hiroto Kita, Yong Jun Liu, Aftab A. Ansari, Kentaro Kikuchi, Susumu Ikehara, M. Eric Gershwin

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Abstract

Liver dendritic cells (DC) are believed to play important roles in liver immunity, autoimmunity, and in the regulation of hepatic allograft acceptance. However, limited information is available on the phenotypes and functions of DC in the liver. To address this issue, we isolated DC from murine liver using procedures that do not involve collagenase, and characterized the freshly isolated DC population that had not been subjected to in vitro expansion. Thence, based on the expression of CD4, B220, and CD11b, four subsets or groups of hepatic NK1.1-CD11c+ DC were identified with the following phenotypes: B220+CD4+, B220+CD4-, B220-CD11b+, and B220-CD11b-. Each subset was further characterized both phenotypically and functionally. In addition to unique phenotypic expression, each subset displayed different allostimulation capability in mixed lymphocyte reaction assays. All four groups developed DC morphology following in vitro culture with activation agents and synthesized distinct patterns of cytokines in response to different stimuli. Taken together, our results suggest that groups I and II are IFN-α-producing plasmacytoid DC, group III cells are myeloid-related DC, while group IV is a heterogenous population containing both myeloid- and lymphoid-related DC. Our results demonstrate the highly heterogeneous nature of hepatic DC, which is in agreement with the unique requirements for APC in the complex liver environment.

Original languageEnglish (US)
Pages (from-to)2323-2330
Number of pages8
JournalJournal of Immunology
Volume170
Issue number5
StatePublished - Mar 1 2003

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Dendritic Cells
Liver
Population
Phenotype
Mixed Lymphocyte Culture Test
Collagenases
Myeloid Cells
Autoimmunity
Allografts
Hepatocytes
Immunity
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Heterogeneity of dendritic cells in the mouse liver : Identification and characterization of four distinct populations. / Lian, Zhe Xiong; Okada, Tomoyuki; He, Xiaosong; Kita, Hiroto; Liu, Yong Jun; Ansari, Aftab A.; Kikuchi, Kentaro; Ikehara, Susumu; Gershwin, M. Eric.

In: Journal of Immunology, Vol. 170, No. 5, 01.03.2003, p. 2323-2330.

Research output: Contribution to journalArticle

Lian, ZX, Okada, T, He, X, Kita, H, Liu, YJ, Ansari, AA, Kikuchi, K, Ikehara, S & Gershwin, ME 2003, 'Heterogeneity of dendritic cells in the mouse liver: Identification and characterization of four distinct populations', Journal of Immunology, vol. 170, no. 5, pp. 2323-2330.
Lian, Zhe Xiong ; Okada, Tomoyuki ; He, Xiaosong ; Kita, Hiroto ; Liu, Yong Jun ; Ansari, Aftab A. ; Kikuchi, Kentaro ; Ikehara, Susumu ; Gershwin, M. Eric. / Heterogeneity of dendritic cells in the mouse liver : Identification and characterization of four distinct populations. In: Journal of Immunology. 2003 ; Vol. 170, No. 5. pp. 2323-2330.
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AU - Liu, Yong Jun

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