Heterogeneity in phagocytic and nitroblue tetrazolium reductive properties of neutrophils from cows.

M. B. Kabbur, N. C. Jain, J. G. Zinkl, Thomas B Farver

Research output: Contribution to journalArticle

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Abstract

Phagocytic and nitroblue tetrazolium (NBT) reductive activities of blood neutrophils from 19 Holstein heifers were measured by light microscopic and spectrophotometric methods, respectively. These functional properties of neutrophils correlated well (r = 0.64) and varied significantly (P less than 0.05) among animals studied. Variations in phagocytosis and NBT reductive activities attributable to the source of sera were determined in experiments in which cells from the same cows and zymogen particles opsonized with heat-inactivated autologous or homologous sera were used. Variations attributable to the source of cells were determined in experiments in which cells from different cows and particles opsonized with pooled sera from all the cows were used. Most of the variation in phagocytic properties and NBT reductive activities was attributable to the source of cells (ie, each cow). The source of sera contributed slightly to the variation in NBT reductive activities, but not to the phagocytic properties. These results support the concept of functional heterogeneity of neutrophils among cows.

Original languageEnglish (US)
Pages (from-to)2023-2028
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume52
Issue number12
StatePublished - Dec 1991

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nitroblue tetrazolium
Nitroblue Tetrazolium
neutrophils
blood serum
Neutrophils
cows
Serum
Enzyme Precursors
cells
zymogens
Phagocytosis
phagocytosis
Hot Temperature
functional properties
heifers
Light
Holstein
heat
blood
animals

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Heterogeneity in phagocytic and nitroblue tetrazolium reductive properties of neutrophils from cows. / Kabbur, M. B.; Jain, N. C.; Zinkl, J. G.; Farver, Thomas B.

In: American Journal of Veterinary Research, Vol. 52, No. 12, 12.1991, p. 2023-2028.

Research output: Contribution to journalArticle

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