Herpes simplex virus type 1 glycoprotein gC mediates immune evasion in vivo

John M. Lubinski, Liyang Wang, Athena Soulika, Reinhard Burger, Rick A. Wetsel, Harvey Colten, Gary H. Cohen, Roselyn J. Eisenberg, John D. Lambris, Harvey M. Friedman

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Many microorganisms encode proteins that interact with molecules involved in host immunity; however, few of these molecules have been proven to promote immune evasion in vivo. Herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) binds complement component C3 and inhibits complement- mediated virus neutralization and lysis of infected cells in vitro. To investigate the importance of the interaction between gC and C3 in vivo, we studied the virulence of a gC-null strain in complement-intact and C3- deficient animals. Using a vaginal infection model in complement-intact guinea pigs, we showed that gC-null virus grows to lower titers and produces less severe vaginitis than wild-type or gC rescued virus, indicating a role for gC in virulence. To determine the importance of complement, studies were performed with C3-deficient guinea pigs; the results demonstrated significant increases in vaginal titers of gC-null virus, while wild-type and gC rescued viruses showed nonsignificant changes in titers. Similar findings were observed for mice where gC null virus produced significantly less disease than gC rescued virus at the skin inoculation site. Proof that C3 is important was provided by studies of C3 knockout mice, where disease scores of gC-null virus were significantly higher than in complement-intact mice. The results indicate that gC-null virus is approximately 100-fold (2 log10) less virulent that wild-type virus in animals and that gC-C3 interactions are involved in pathogenesis.

Original languageEnglish (US)
Pages (from-to)8257-8263
Number of pages7
JournalJournal of Virology
Volume72
Issue number10
StatePublished - Oct 1998
Externally publishedYes

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immune evasion
Immune Evasion
Human herpesvirus 1
Human Herpesvirus 1
glycoproteins
Glycoproteins
Viruses
viruses
complement
Complement C3
glycoprotein gC, herpes simplex virus type 1
guinea pigs
Virulence
mice
Guinea Pigs
virulence
vaginitis
Vaginitis
neutralization tests
skin (animal)

ASJC Scopus subject areas

  • Immunology

Cite this

Lubinski, J. M., Wang, L., Soulika, A., Burger, R., Wetsel, R. A., Colten, H., ... Friedman, H. M. (1998). Herpes simplex virus type 1 glycoprotein gC mediates immune evasion in vivo. Journal of Virology, 72(10), 8257-8263.

Herpes simplex virus type 1 glycoprotein gC mediates immune evasion in vivo. / Lubinski, John M.; Wang, Liyang; Soulika, Athena; Burger, Reinhard; Wetsel, Rick A.; Colten, Harvey; Cohen, Gary H.; Eisenberg, Roselyn J.; Lambris, John D.; Friedman, Harvey M.

In: Journal of Virology, Vol. 72, No. 10, 10.1998, p. 8257-8263.

Research output: Contribution to journalArticle

Lubinski, JM, Wang, L, Soulika, A, Burger, R, Wetsel, RA, Colten, H, Cohen, GH, Eisenberg, RJ, Lambris, JD & Friedman, HM 1998, 'Herpes simplex virus type 1 glycoprotein gC mediates immune evasion in vivo', Journal of Virology, vol. 72, no. 10, pp. 8257-8263.
Lubinski JM, Wang L, Soulika A, Burger R, Wetsel RA, Colten H et al. Herpes simplex virus type 1 glycoprotein gC mediates immune evasion in vivo. Journal of Virology. 1998 Oct;72(10):8257-8263.
Lubinski, John M. ; Wang, Liyang ; Soulika, Athena ; Burger, Reinhard ; Wetsel, Rick A. ; Colten, Harvey ; Cohen, Gary H. ; Eisenberg, Roselyn J. ; Lambris, John D. ; Friedman, Harvey M. / Herpes simplex virus type 1 glycoprotein gC mediates immune evasion in vivo. In: Journal of Virology. 1998 ; Vol. 72, No. 10. pp. 8257-8263.
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