Hepatocyte RXR alpha deletion in mice leads to inhibition of angiogenesis

Urszula Razny, Lukasz Wator, Anna Polus, Yu-Jui Yvonne Wan, Grzegorz Dyduch, Romana Tomaszewska, Aldona Dembinska-Kiec

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To investigate the effect of RXRα deficiency in liver on angiogenesis, hepatocyte RXRα-deficient and control wild-type mice were fed either standard or high-fat diet (HF) for 7 weeks. In the 6th week of feeding, Matrigel model of in vivo angiogenesis was applied. Matrigel plug infiltrating cells were then used for visualization of vessels (CD31 staining) as well as for gene expression analysis. HF diet appeared to decrease angiogenesis in hRXRα-deficient mice. Genes related to angiogenesis (Nos3, Kdr) were down-regulated, whereas genes connected with adipogenesis (Cebpb, Srebf1), apoptosis (Gzmb, Bcl2) and proinflammatory pathway (NfκB, Tnfα) were up-regulated by HF diet in hRXRα-deficient mice in the microarray study. Our results suggest that impaired fatty acid metabolism in liver (hRXRα-deficient mice) leads to impaired angiogenesis due to lipotoxicity and promotion of adipogenesis.

Original languageEnglish (US)
Pages (from-to)69-72
Number of pages4
JournalGenes and Nutrition
Volume4
Issue number1
DOIs
StatePublished - Mar 2009
Externally publishedYes

Keywords

  • Angiogenesis
  • FFA
  • Keywords Adipogenesis
  • Retinoid X receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Genetics

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  • Cite this

    Razny, U., Wator, L., Polus, A., Wan, Y-J. Y., Dyduch, G., Tomaszewska, R., & Dembinska-Kiec, A. (2009). Hepatocyte RXR alpha deletion in mice leads to inhibition of angiogenesis. Genes and Nutrition, 4(1), 69-72. https://doi.org/10.1007/s12263-009-0111-z