Hepatic gene expression after direct DNA injection

M. Anne Hickman, Robert W. Malone, Tracey R. Sih, Geoffrey Y. Akita, Don M. Carlson, Jerry S Powell

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The liver is an attractive target tissue for gene therapy. Current techniques which may be useful for in vivo hepatic gene delivery include viral vectors such as retroviruses, adenoviruses and adeno-associated viruses, and non-viral methods including liposome/DNA complexes, peptide/DNA complexes, dendrimer delivery and direct injection of DNA. While, previous studies have suggested that genes cannot be delivered to liver tissue by direct DNA injection, this review focuses on the factors that lead to significant gene expression in liver, as compared to other tissues, after direct injection of plasmid DNA. Several factors are discussed, including injection technique, DNA dose, DNA diluent and enhancement of transfected gene expression by dexamethasone administration. The current limitations and potential uses of direct DNA injection into liver are reviewed. Direct DNA injection is currently a reliable, reproducible and simple alternative for hepatic gene transduction that provides a tool for molecular research and may have future potential for therapeutic gene delivery.

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalAdvanced Drug Delivery Reviews
Volume17
Issue number3
DOIs
StatePublished - Dec 5 1995

Fingerprint

Gene Expression
Injections
Liver
DNA
Genes
Dendrimers
Dependovirus
Retroviridae
Cell- and Tissue-Based Therapy
Adenoviridae
Liposomes
Genetic Therapy
Dexamethasone
Plasmids
Peptides
Research

Keywords

  • Dexamethasone
  • Erythropoietin
  • Gene therapy
  • Gene transfer
  • Liver
  • Luciferase
  • Plasmid expression

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Hickman, M. A., Malone, R. W., Sih, T. R., Akita, G. Y., Carlson, D. M., & Powell, J. S. (1995). Hepatic gene expression after direct DNA injection. Advanced Drug Delivery Reviews, 17(3), 265-271. https://doi.org/10.1016/0169-409X(95)00060-K

Hepatic gene expression after direct DNA injection. / Hickman, M. Anne; Malone, Robert W.; Sih, Tracey R.; Akita, Geoffrey Y.; Carlson, Don M.; Powell, Jerry S.

In: Advanced Drug Delivery Reviews, Vol. 17, No. 3, 05.12.1995, p. 265-271.

Research output: Contribution to journalArticle

Hickman, MA, Malone, RW, Sih, TR, Akita, GY, Carlson, DM & Powell, JS 1995, 'Hepatic gene expression after direct DNA injection', Advanced Drug Delivery Reviews, vol. 17, no. 3, pp. 265-271. https://doi.org/10.1016/0169-409X(95)00060-K
Hickman, M. Anne ; Malone, Robert W. ; Sih, Tracey R. ; Akita, Geoffrey Y. ; Carlson, Don M. ; Powell, Jerry S. / Hepatic gene expression after direct DNA injection. In: Advanced Drug Delivery Reviews. 1995 ; Vol. 17, No. 3. pp. 265-271.
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