Abstract
We measured 16 nonesterified oxygenated fatty acid derivatives (oxylipids) in plasmas from seven human subjects. Two arterial samples from each subject were analyzed, drawn approximately 2 h apart. We observed a marked increase in levels of most oxylipids in the second sample, as high as 470-fold. Between the first and second samples, subjects received approximately 800-1000 IU of heparin to prevent clotting in intravascular catheters. We postulate that heparin activated lipoprotein lipases, which, in turn, released oxylipids from triglycerides and phospholipids in plasma lipoproteins. Some of that lipolysis may have occurred during sample storage. Measurements of nonesterified lipids in human plasma may be distorted if heparin is administered to subjects before blood is drawn and if lipase inhibitors are omitted from stored samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 363-366 |
| Number of pages | 4 |
| Journal | Prostaglandins Leukotrienes and Essential Fatty Acids |
| Volume | 77 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - Nov 2007 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Endocrinology, Diabetes and Metabolism
Cite this
Heparin, lipoproteins, and oxygenated fatty acids in blood : A cautionary note. / Goodfriend, T. L.; Pedersen, T. L.; Grekin, R. J.; Hammock, B. D.; Ball, D. L.; Vollmer, A.
In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 77, No. 5-6, 11.2007, p. 363-366.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heparin, lipoproteins, and oxygenated fatty acids in blood
T2 - A cautionary note
AU - Goodfriend, T. L.
AU - Pedersen, T. L.
AU - Grekin, R. J.
AU - Hammock, B. D.
AU - Ball, D. L.
AU - Vollmer, A.
PY - 2007/11
Y1 - 2007/11
N2 - We measured 16 nonesterified oxygenated fatty acid derivatives (oxylipids) in plasmas from seven human subjects. Two arterial samples from each subject were analyzed, drawn approximately 2 h apart. We observed a marked increase in levels of most oxylipids in the second sample, as high as 470-fold. Between the first and second samples, subjects received approximately 800-1000 IU of heparin to prevent clotting in intravascular catheters. We postulate that heparin activated lipoprotein lipases, which, in turn, released oxylipids from triglycerides and phospholipids in plasma lipoproteins. Some of that lipolysis may have occurred during sample storage. Measurements of nonesterified lipids in human plasma may be distorted if heparin is administered to subjects before blood is drawn and if lipase inhibitors are omitted from stored samples.
AB - We measured 16 nonesterified oxygenated fatty acid derivatives (oxylipids) in plasmas from seven human subjects. Two arterial samples from each subject were analyzed, drawn approximately 2 h apart. We observed a marked increase in levels of most oxylipids in the second sample, as high as 470-fold. Between the first and second samples, subjects received approximately 800-1000 IU of heparin to prevent clotting in intravascular catheters. We postulate that heparin activated lipoprotein lipases, which, in turn, released oxylipids from triglycerides and phospholipids in plasma lipoproteins. Some of that lipolysis may have occurred during sample storage. Measurements of nonesterified lipids in human plasma may be distorted if heparin is administered to subjects before blood is drawn and if lipase inhibitors are omitted from stored samples.
UR - http://www.scopus.com/inward/record.url?scp=36649025516&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36649025516&partnerID=8YFLogxK
U2 - 10.1016/j.plefa.2007.10.012
DO - 10.1016/j.plefa.2007.10.012
M3 - Article
C2 - 18036802
AN - SCOPUS:36649025516
VL - 77
SP - 363
EP - 366
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
IS - 5-6
ER -