Heparin hydrogel sandwich cultures of primary hepatocytes

Elena Foster, Jungmok You, Christian Siltanen, Dipali Patel, Amranul Haque, Leif Anderson, Alexander Revzin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Long-term in vitro maintenance of primary hepatocytes has been a challenge in hepatic tissue engineering, since these cells dedifferentiate and lose their phenotype in standard culture conditions. Some of the most successful culture systems employ collagen or Matrigel matrices. As an alternative matrix, our laboratory has been exploring the use of heparin-containing hydrogels as scaffolds for cultivation of functional hepatocytes. The present study characterized a heparin gel sandwich culture system for maintenance of primary rat hepatocytes. The heparin gel layers were fabricated via UV light induced thiol-ene coupling reaction of thiolated heparin (Hep-SH) and diacrylated poly(ethylene glycol) (PEG-DA) precursors. Analysis of hepatic function revealed that cells sustained albumin secretion for at least three weeks at similarly high levels for collagen and heparin gel sandwich culture systems. Furthermore, cytochrome P450 activity of hepatocytes was ∼1.6 times higher in heparin gels compared to collagen gels. Another distinct advantage of heparin is its ability to bind and release growth factors in a controlled manner. We observed that after 24 days hepatocytes cultured in heparin gel sandwich containing hepatocyte growth factor (HGF) produced ∼1.8 times higher amounts of albumin compared to cells in collagen sandwiches. Overall, we demonstrate heparin gel sandwich to be an excellent system for maintaining differentiated, polarized primary hepatocytes for over three weeks. The biomaterial used in this study, heparin-PEG hydrogel, offers significant flexibility in terms of tuning mechanical properties, adjusting heparin content or introducing photolabile chemistries to promote gel degradation. We see multiple applications for this novel system in hepatocyte maintenance and stem cell differentiation studies.

Original languageEnglish (US)
Pages (from-to)726-735
Number of pages10
JournalEuropean Polymer Journal
Volume72
DOIs
StatePublished - Jan 2 2015

Fingerprint

heparins
Hydrogel
Hydrogels
Heparin
Gels
gels
collagens
Collagen
Polyethylene glycols
maintenance
cells
albumins
Albumins
Hepatocytes
secretions
phenotype
Hepatocyte Growth Factor
stem cells
tissue engineering
cytochromes

Keywords

  • 3D polymer matrix
  • Heparin hydrogel
  • Hepatocyte
  • Hepatocyte growth factor
  • Polarity
  • Sandwich culture

ASJC Scopus subject areas

  • Polymers and Plastics
  • Physics and Astronomy(all)
  • Organic Chemistry

Cite this

Foster, E., You, J., Siltanen, C., Patel, D., Haque, A., Anderson, L., & Revzin, A. (2015). Heparin hydrogel sandwich cultures of primary hepatocytes. European Polymer Journal, 72, 726-735. https://doi.org/10.1016/j.eurpolymj.2014.12.033

Heparin hydrogel sandwich cultures of primary hepatocytes. / Foster, Elena; You, Jungmok; Siltanen, Christian; Patel, Dipali; Haque, Amranul; Anderson, Leif; Revzin, Alexander.

In: European Polymer Journal, Vol. 72, 02.01.2015, p. 726-735.

Research output: Contribution to journalArticle

Foster, E, You, J, Siltanen, C, Patel, D, Haque, A, Anderson, L & Revzin, A 2015, 'Heparin hydrogel sandwich cultures of primary hepatocytes', European Polymer Journal, vol. 72, pp. 726-735. https://doi.org/10.1016/j.eurpolymj.2014.12.033
Foster E, You J, Siltanen C, Patel D, Haque A, Anderson L et al. Heparin hydrogel sandwich cultures of primary hepatocytes. European Polymer Journal. 2015 Jan 2;72:726-735. https://doi.org/10.1016/j.eurpolymj.2014.12.033
Foster, Elena ; You, Jungmok ; Siltanen, Christian ; Patel, Dipali ; Haque, Amranul ; Anderson, Leif ; Revzin, Alexander. / Heparin hydrogel sandwich cultures of primary hepatocytes. In: European Polymer Journal. 2015 ; Vol. 72. pp. 726-735.
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