Hemotropic mycoplasmas in little brown bats (Myotis lucifugus)

Patricia E. Mascarelli, Michael K Keel, Michael Yabsley, Lisa A. Last, Edward B. Breitschwerdt, Ricardo G. Maggi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Hemotropic mycoplasmas are epicellular erythrocytic bacteria that can cause infectious anemia in some mammalian species. Worldwide, hemotropic mycoplasmas are emerging or re-emerging zoonotic pathogens potentially causing serious and significant health problems in wildlife. The objective of this study was to determine the molecular prevalence of hemotropic Mycoplasma species in little brown bats (Myotis lucifugus) with and without Pseudogymnoascus (Geomyces) destrucans, the causative agent of white nose syndrome (WNS) that causes significant mortality events in bats. Methods. In order to establish the prevalence of hemotropic Mycoplasma species in a population of 68 little brown bats (Myotis lucifugus) with (n = 53) and without (n = 15) white-nose syndrome (WNS), PCR was performed targeting the 16S rRNA gene. Results: The overall prevalence of hemotropic Mycoplasmas in bats was 47%, with similar (p = 0.5725) prevalence between bats with WNS (49%) and without WNS (40%). 16S rDNA sequence analysis (∼1,200 bp) supports the presence of a novel hemotropic Mycoplasma species with 91.75% sequence homology with Mycoplasma haemomuris. No differences were found in gene sequences generated from WNS and non-WNS animals. Conclusions: Gene sequences generated from WNS and non-WNS animals suggest that little brown bats could serve as a natural reservoir for this potentially novel Mycoplasma species. Currently, there is minimal information about the prevalence, host-specificity, or the route of transmission of hemotropic Mycoplasma spp. among bats. Finally, the potential role of hemotropic Mycoplasma spp. as co-factors in the development of disease manifestations in bats, including WNS in Myotis lucifugus, remains to be elucidated.

Original languageEnglish (US)
Article number117
JournalParasites and Vectors
Volume7
Issue number1
DOIs
StatePublished - Mar 24 2014

Fingerprint

Mycoplasma
Nose
Host Specificity
Zoonoses
Sequence Homology
Ribosomal DNA
rRNA Genes
Genes
Sequence Analysis
Anemia
Bacteria
Polymerase Chain Reaction
Mortality

Keywords

  • Bat
  • Haemoplasma
  • Hemotropic mycoplasma
  • Mycplasma
  • WNS

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Mascarelli, P. E., Keel, M. K., Yabsley, M., Last, L. A., Breitschwerdt, E. B., & Maggi, R. G. (2014). Hemotropic mycoplasmas in little brown bats (Myotis lucifugus). Parasites and Vectors, 7(1), [117]. https://doi.org/10.1186/1756-3305-7-117

Hemotropic mycoplasmas in little brown bats (Myotis lucifugus). / Mascarelli, Patricia E.; Keel, Michael K; Yabsley, Michael; Last, Lisa A.; Breitschwerdt, Edward B.; Maggi, Ricardo G.

In: Parasites and Vectors, Vol. 7, No. 1, 117, 24.03.2014.

Research output: Contribution to journalArticle

Mascarelli, PE, Keel, MK, Yabsley, M, Last, LA, Breitschwerdt, EB & Maggi, RG 2014, 'Hemotropic mycoplasmas in little brown bats (Myotis lucifugus)', Parasites and Vectors, vol. 7, no. 1, 117. https://doi.org/10.1186/1756-3305-7-117
Mascarelli PE, Keel MK, Yabsley M, Last LA, Breitschwerdt EB, Maggi RG. Hemotropic mycoplasmas in little brown bats (Myotis lucifugus). Parasites and Vectors. 2014 Mar 24;7(1). 117. https://doi.org/10.1186/1756-3305-7-117
Mascarelli, Patricia E. ; Keel, Michael K ; Yabsley, Michael ; Last, Lisa A. ; Breitschwerdt, Edward B. ; Maggi, Ricardo G. / Hemotropic mycoplasmas in little brown bats (Myotis lucifugus). In: Parasites and Vectors. 2014 ; Vol. 7, No. 1.
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abstract = "Background: Hemotropic mycoplasmas are epicellular erythrocytic bacteria that can cause infectious anemia in some mammalian species. Worldwide, hemotropic mycoplasmas are emerging or re-emerging zoonotic pathogens potentially causing serious and significant health problems in wildlife. The objective of this study was to determine the molecular prevalence of hemotropic Mycoplasma species in little brown bats (Myotis lucifugus) with and without Pseudogymnoascus (Geomyces) destrucans, the causative agent of white nose syndrome (WNS) that causes significant mortality events in bats. Methods. In order to establish the prevalence of hemotropic Mycoplasma species in a population of 68 little brown bats (Myotis lucifugus) with (n = 53) and without (n = 15) white-nose syndrome (WNS), PCR was performed targeting the 16S rRNA gene. Results: The overall prevalence of hemotropic Mycoplasmas in bats was 47{\%}, with similar (p = 0.5725) prevalence between bats with WNS (49{\%}) and without WNS (40{\%}). 16S rDNA sequence analysis (∼1,200 bp) supports the presence of a novel hemotropic Mycoplasma species with 91.75{\%} sequence homology with Mycoplasma haemomuris. No differences were found in gene sequences generated from WNS and non-WNS animals. Conclusions: Gene sequences generated from WNS and non-WNS animals suggest that little brown bats could serve as a natural reservoir for this potentially novel Mycoplasma species. Currently, there is minimal information about the prevalence, host-specificity, or the route of transmission of hemotropic Mycoplasma spp. among bats. Finally, the potential role of hemotropic Mycoplasma spp. as co-factors in the development of disease manifestations in bats, including WNS in Myotis lucifugus, remains to be elucidated.",
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