Hemolytic complement measurement in eleven species of nonhuman primates

L. R. Ellingsworth, C. A. Holmberg, Bennie Osburn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A microtiter system was used to measure hemolytic complement levels in serum from eleven nonhuman primate species. The species studied were Macaca mulatta (rhesus macaque), Macaca radiata (bonnet macaque), Macaca nemestrina (pig-tailed macaque), Macaca fascicularis (crab-eating macaque), Macaca speciosa (stumptailed macaque), Papio cynocephalus (yellow baboon), Papio anubis (olive baboon), Cercopithecus aethiops (African green monkey), Aotus trivirgatus (owl monkey), Ateles fusceps robustus (spider monkey), and Galago crassicaudatus panganiensis (thick-tailed galago). The optimal hemolytic complement titer of the various nonhuman primate species was found to vary with different species sources of erythrocytes and anti-erythrocyte reagents used in the assay. No single erythrocyte and anti-erythrocyte test reagent produced optimal titers for all of the primate species examined. Sera from several species was found to have high spontaneous lytic activity towards non-sensitized sheep erythrocytes which for six species (M. mulatta, M. radiata, M. speciosa, P. cynocephalus, P. anubis and A. trivirgatus) was equal to the titer for antibody sensitized erythrocytes. Evidence of alternate pathway complement activation as a possible reason for the high titer of lytic activity towards unsensitized erythrocytes could not be demonstrated for any nonhuman primate species. In one species, M. mulatta, the sensitizing activity of normal serum for sheep erythrocytes was shown to be in the IgM containing fraction obtained with gel filtration and to be absorbed by boiled sheep erythrocyte stroma which contains Forssman antigen.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume5
Issue number2
DOIs
StatePublished - Jan 1 1983

Fingerprint

Primates
Complement System Proteins
complement
erythrocytes
Erythrocytes
Papio cynocephalus
Macaca radiata
Papio anubis
Macaca mulatta
Aotus trivirgatus
Macaca
Galago
Atelinae
Macaca nemestrina
Aotus (Cebidae)
Cercopithecus aethiops
Sheep
Macaca fascicularis
sheep
Forssman Antigen

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Hemolytic complement measurement in eleven species of nonhuman primates. / Ellingsworth, L. R.; Holmberg, C. A.; Osburn, Bennie.

In: Veterinary Immunology and Immunopathology, Vol. 5, No. 2, 01.01.1983, p. 141-149.

Research output: Contribution to journalArticle

Ellingsworth, L. R. ; Holmberg, C. A. ; Osburn, Bennie. / Hemolytic complement measurement in eleven species of nonhuman primates. In: Veterinary Immunology and Immunopathology. 1983 ; Vol. 5, No. 2. pp. 141-149.
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