Heme orientational heterogeneity in deuterohemin-reconstituted horse and human hemoglobin characterized by proton nuclear magnetic resonance spectroscopy

Thomas Jue, Gerd N. La Mar

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The number of 2,4-H signals of met-cyano and deoxy deuteroheme-reconstituted sperm whale Mb are shown to reflect the known degree of heme rotational disorder in this modified protein. Using these unique spectral windows for the 2,4-H signals, we show that both horse and human Hb reconstituted with deuteroheme exhibit significant molecular heterogeneity which is consistent with approximately 20% heme rotational disorder within each subunit.

Original languageEnglish (US)
Pages (from-to)640-645
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume119
Issue number2
DOIs
StatePublished - Mar 15 1984

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Heme orientational heterogeneity in deuterohemin-reconstituted horse and human hemoglobin characterized by proton nuclear magnetic resonance spectroscopy'. Together they form a unique fingerprint.

  • Cite this