Abstract
The number of 2,4-H signals of met-cyano and deoxy deuteroheme-reconstituted sperm whale Mb are shown to reflect the known degree of heme rotational disorder in this modified protein. Using these unique spectral windows for the 2,4-H signals, we show that both horse and human Hb reconstituted with deuteroheme exhibit significant molecular heterogeneity which is consistent with approximately 20% heme rotational disorder within each subunit.
Original language | English (US) |
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Pages (from-to) | 640-645 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 119 |
Issue number | 2 |
DOIs | |
State | Published - Mar 15 1984 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology