The number of 2,4-H signals of met-cyano and deoxy deuteroheme-reconstituted sperm whale Mb are shown to reflect the known degree of heme rotational disorder in this modified protein. Using these unique spectral windows for the 2,4-H signals, we show that both horse and human Hb reconstituted with deuteroheme exhibit significant molecular heterogeneity which is consistent with approximately 20% heme rotational disorder within each subunit.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 15 1984|
ASJC Scopus subject areas
- Molecular Biology