Abstract
The number of 2,4-H signals of met-cyano and deoxy deuteroheme-reconstituted sperm whale Mb are shown to reflect the known degree of heme rotational disorder in this modified protein. Using these unique spectral windows for the 2,4-H signals, we show that both horse and human Hb reconstituted with deuteroheme exhibit significant molecular heterogeneity which is consistent with approximately 20% heme rotational disorder within each subunit.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 640-645 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 119 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 15 1984 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Jue, T., & La Mar, G. N. (1984). Heme orientational heterogeneity in deuterohemin-reconstituted horse and human hemoglobin characterized by proton nuclear magnetic resonance spectroscopy. Biochemical and Biophysical Research Communications, 119(2), 640-645. https://doi.org/10.1016/S0006-291X(84)80297-1