Hematopoietic progenitor cell regulation by CD4+CD25+ T cells

Maite Urbieta, Isabel Barao, Monica Jones, Roland Jurecic, Angela Panoskaltsis-Mortari, Bruce R. Blazar, William J Murphy, Robert B. Levy

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

CD4+CD25+FoxP3+ regulatory T cells (Tregs) possess the capacity to modulate both adaptive and innate immune responses. We hypothesized that Tregs could regulate hematopoiesis based on cytokine effector molecules they can produce. The studies here demonstrate that Tregs can affect the differentiation of myeloid progenitor cells. In vitro findings demonstrated the ability of Tregs to inhibit the differentiation of interleukin-3 (IL-3)/stem cell factor (colony-forming unit [CFU]-IL3)-driven progenitor cells. Inhibitory effects were mediated by a pathway requiring cell-cell contact, major histocompatibility complex class II expression on marrow cells, and transforming growth factor-β. Importantly, depletion of Tregs in situ resulted in enhanced CFU-IL3 levels after bone marrow transplantation. Cotransplantation of CD4+ FoxP3+gfp Tregs together with bone marrow was found to diminish CFU-IL3 responses after transplantation. To address the consequence of transplanted Tregs on differentiated progeny from these CFU 2 weeks after hematopoietic stem cell transplantation, peripheral blood complete blood counts were performed and examined for polymorphonuclear leukocyte content. Recipients of cotransplanted Tregs exhibited diminished neutrophil counts. Together, these findings illustrate that both recipient and donor Tregs can influence hematopoietic progenitor cell activity after transplantation and that these cells can alter responses outside the adaptive and innate immune systems.

Original languageEnglish (US)
Pages (from-to)4934-4943
Number of pages10
JournalBlood
Volume115
Issue number23
DOIs
StatePublished - Jun 10 2010
Externally publishedYes

Fingerprint

T-cells
Hematopoietic Stem Cells
Bone
Blood
Stem Cells
T-Lymphocytes
Stem Cell Factor
Interleukin-3
Immune system
Transforming Growth Factors
Stem cells
Cells
Cytokines
Neutrophils
Bone Marrow
Molecules
Myeloid Progenitor Cells
Blood Cell Count
Hematopoietic Stem Cell Transplantation
Cell Transplantation

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Urbieta, M., Barao, I., Jones, M., Jurecic, R., Panoskaltsis-Mortari, A., Blazar, B. R., ... Levy, R. B. (2010). Hematopoietic progenitor cell regulation by CD4+CD25+ T cells. Blood, 115(23), 4934-4943. https://doi.org/10.1182/blood-2009-04-218826

Hematopoietic progenitor cell regulation by CD4+CD25+ T cells. / Urbieta, Maite; Barao, Isabel; Jones, Monica; Jurecic, Roland; Panoskaltsis-Mortari, Angela; Blazar, Bruce R.; Murphy, William J; Levy, Robert B.

In: Blood, Vol. 115, No. 23, 10.06.2010, p. 4934-4943.

Research output: Contribution to journalArticle

Urbieta, M, Barao, I, Jones, M, Jurecic, R, Panoskaltsis-Mortari, A, Blazar, BR, Murphy, WJ & Levy, RB 2010, 'Hematopoietic progenitor cell regulation by CD4+CD25+ T cells', Blood, vol. 115, no. 23, pp. 4934-4943. https://doi.org/10.1182/blood-2009-04-218826
Urbieta M, Barao I, Jones M, Jurecic R, Panoskaltsis-Mortari A, Blazar BR et al. Hematopoietic progenitor cell regulation by CD4+CD25+ T cells. Blood. 2010 Jun 10;115(23):4934-4943. https://doi.org/10.1182/blood-2009-04-218826
Urbieta, Maite ; Barao, Isabel ; Jones, Monica ; Jurecic, Roland ; Panoskaltsis-Mortari, Angela ; Blazar, Bruce R. ; Murphy, William J ; Levy, Robert B. / Hematopoietic progenitor cell regulation by CD4+CD25+ T cells. In: Blood. 2010 ; Vol. 115, No. 23. pp. 4934-4943.
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