Hematologic and growth-related effects of frequent prenatal ultrasound exposure in the long-tailed macaque (Macaca fascicularis)

Alice F Tarantal, S. E. Gargosky, D. S. Ellis, W. D. O'Brien, Andrew G Hendrickx

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Prior investigations have shown that reduced birth weights and transient neutropenias result from frequent exposure of monkey fetuses to ultrasound. To further explore these findings, 26 animals were studied (16 exposed, 10 controls; "triple mode"; ATL Ultramark 9 with HDI®; ISPTAd ∼ 645 to 714 mW/cm2). Exposures were performed daily for 5 days each week from gestational days (GD) 21 to 35 (5 min), three times weekly from GD 36 to 60 (5 min), then weekly from GD 61 to 153 ± 1 (10 min). Fetal blood samples (FBS) were collected for complete blood counts (CBCs), hematopoietic progenitor assay, circulating insulin-like growth factors (IGF-I, IGF-II) and binding proteins (IGFBP-3) (GD 120, 140, 153 ± 1). Animals were delivered by Cesarean section at term (GD 153 ± 1), and body weights, morphometrics, CBCs, and bone marrow aspirates assessed at delivery and postnatally for 3 months. Fetal neutropenias were noted in exposed animals in addition to reduced circulating progenitors (colony forming unit-granulocytemacrophage [CFU-GM]). Growth of CFU-GM from bone marrow was exuberant at term, whereas circulating levels were diminished comparable to prenatal samples. Exposed animals were smaller at birth; marked reductions in IGFBP-3 were noted prenatally. These data suggest that frequent prenatal ultrasound exposure can transiently alter the neutrophil lineage, although these findings may be the result of enhanced margination and organ sequestration. Data also suggest that transient, altered growth patterns may be due to perturbations of the IGF axis.

Original languageEnglish (US)
Pages (from-to)1073-1081
Number of pages9
JournalUltrasound in Medicine and Biology
Volume21
Issue number8
DOIs
StatePublished - 1995

Fingerprint

Macaca fascicularis
Macaca
animals
blood
Insulin-Like Growth Factor Binding Protein 3
Blood Cell Count
bone marrow
Growth
Neutropenia
Insulin-Like Growth Factor I
Stem Cells
Bone Marrow
neutrophils
monkeys
body weight
Insulin-Like Growth Factor Binding Proteins
insulin
Insulin-Like Growth Factor II
fetuses
Fetal Blood

Keywords

  • Bioeffects
  • Fetus
  • Growth
  • Hematopoiesis
  • IGF
  • Monkey
  • Sonography

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Hematologic and growth-related effects of frequent prenatal ultrasound exposure in the long-tailed macaque (Macaca fascicularis). / Tarantal, Alice F; Gargosky, S. E.; Ellis, D. S.; O'Brien, W. D.; Hendrickx, Andrew G.

In: Ultrasound in Medicine and Biology, Vol. 21, No. 8, 1995, p. 1073-1081.

Research output: Contribution to journalArticle

Tarantal, Alice F ; Gargosky, S. E. ; Ellis, D. S. ; O'Brien, W. D. ; Hendrickx, Andrew G. / Hematologic and growth-related effects of frequent prenatal ultrasound exposure in the long-tailed macaque (Macaca fascicularis). In: Ultrasound in Medicine and Biology. 1995 ; Vol. 21, No. 8. pp. 1073-1081.
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