Hematologic and biochemical reference intervals for specific pathogen free 6-week-old Hampshire-Yorkshire crossbred pigs

Caitlin A. Cooper, Luis E. Moraes, James D. Murray, Sean D Owens

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Hematologic and biochemical reference intervals depend on many factors, including age. A review of the literature highlights the lack of reference intervals for 6-wk-old specific pathogen free (SPF) Hampshire-Yorkshire crossbred pigs. For translational research, 6-wk-old pigs represent an important animal model for both human juvenile colitis and diabetes mellitus type 2 given the similarities between the porcine and human gastrointestinal maturation process. The aim of this study was to determine reference intervals for hematological and biochemical parameters in healthy 6-wk-old crossbred pigs. Blood samples were collected from 66 clinically healthy Hampshire-Yorkshire pigs. The pigs were 6 wks old, represented both sexes, and were housed in a SPF facility. Automated hematological and biochemical analysis were performed using an ADVIA 120 Hematology System and a Cobas 6000 C501 Clinical Chemistry Analyzer. Results: Reference intervals were calculated using both parametric and nonparametric methods. The mean, median, minimum, and maximum values were calculated. Conclusion: As pigs are used more frequently as medical models of human disease, having reference intervals for commonly measured hematological and biochemical parameters in 6-wk-old pigs will be useful. The reference intervals calculated in this study will aid in the diagnosis and monitoring of both naturally occurring and experimentally induced disease. In comparison to published reference intervals for older non SPF pigs, notable differences in leukocyte populations, and in levels of sodium, potassium, glucose, protein, and alkaline phosphatase were observed.

Original languageEnglish (US)
Article number5
JournalJournal of Animal Science and Biotechnology
Volume5
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Hampshire (swine breed)
Specific Pathogen-Free Organisms
Yorkshire (swine breed)
Pathogens
crossbreds
Swine
swine
pathogens
Phosphoprotein Phosphatases
Medical problems
Alkaline Phosphatase
Potassium
Animals
Blood
Sodium
Glucose
Monitoring
Clinical Chemistry
Translational Medical Research
Age Factors

Keywords

  • Biochemical analytes
  • Hematology
  • Pigs
  • Reference interval
  • SPF

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Biochemistry
  • Animal Science and Zoology

Cite this

Hematologic and biochemical reference intervals for specific pathogen free 6-week-old Hampshire-Yorkshire crossbred pigs. / Cooper, Caitlin A.; Moraes, Luis E.; Murray, James D.; Owens, Sean D.

In: Journal of Animal Science and Biotechnology, Vol. 5, No. 1, 5, 2014.

Research output: Contribution to journalArticle

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