Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner

Fang Yen Li, I. Chun Weng, Chun Hung Lin, Mou Chieh Kao, Ming Shiang Wu, Huan Yuan Chen, Fu-Tong Liu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Galectin-8, a beta-galactoside-binding lectin, is upregulated in the gastric tissues of rhesus macaques infected with Helicobacter pylori. In this study, we found that H. pylori infection triggers intracellular galectin-8 aggregation in human-derived AGS gastric epithelial cells, and that these aggregates colocalize with lysosomes. Notably, this aggregation is markedly reduced following the attenuation of host O-glycan processing. This indicates that H. pylori infection induces lysosomal damage, which in turn results in the accumulation of cytosolic galectin-8 around damaged lysosomes through the recognition of exposed vacuolar host O-glycans. H. pylori-induced galectin-8 aggregates also colocalize with autophagosomes, and galectin-8 ablation reduces the activation of autophagy by H. pylori. This suggests that galectin-8 aggregates may enhance autophagy activity in infected cells. We also observed that both autophagy and NDP52, an autophagy adapter, contribute to the augmentation of galectin-8 aggregation by H. pylori. Additionally, vacuolating cytotoxin A, a secreted H. pylori cytotoxin, may contribute to the increased galectin-8 aggregation and elevated autophagy response in infected cells. Collectively, these results suggest that H. pylori promotes intracellular galectin-8 aggregation, and that galectin-8 aggregation and autophagy may reciprocally regulate each other during infection.

Original languageEnglish (US)
Pages (from-to)151-162
Number of pages12
JournalGlycobiology
Volume29
Issue number2
DOIs
StatePublished - Feb 1 2019

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Galectins
Lysosomes
Helicobacter pylori
Polysaccharides
Stomach
Agglomeration
Epithelial Cells
Autophagy
Cytotoxins
Helicobacter Infections
Ablation
Macaca mulatta

ASJC Scopus subject areas

  • Biochemistry

Cite this

Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner. / Li, Fang Yen; Weng, I. Chun; Lin, Chun Hung; Kao, Mou Chieh; Wu, Ming Shiang; Chen, Huan Yuan; Liu, Fu-Tong.

In: Glycobiology, Vol. 29, No. 2, 01.02.2019, p. 151-162.

Research output: Contribution to journalArticle

Li, Fang Yen ; Weng, I. Chun ; Lin, Chun Hung ; Kao, Mou Chieh ; Wu, Ming Shiang ; Chen, Huan Yuan ; Liu, Fu-Tong. / Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner. In: Glycobiology. 2019 ; Vol. 29, No. 2. pp. 151-162.
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