Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization

Susan K. Amundsen, Jutta Fero, Lori M. Hansen, Gareth A. Cromie, Jay V Solnick, Gerald R. Smith, Nina R. Salama

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Helicobacter pylori colonization of the human stomach is characterized by profound disease-causing inflammation. Bacterial proteins that detoxify reactive oxygen species or recognize damaged DNA adducts promote infection, suggesting that H. pylori requires DNA damage repair for successful in vivo colonization. The molecular mechanisms of repair remain unknown. We identified homologues of the AddAB class of helicase-nuclease enzymes, related to the Escherichia coli RecBCD enzyme, which, with RecA, is required for repair of DNA breaks and homologous recombination. H. pylori mutants lacking addA or addB genes lack detectable ATP-dependent nuclease activity, and the cloned H. pylori addAB genes restore both nuclease and helicase activities to an E. coli recBCD deletion mutant. H. pylori addAB and recA mutants have a reduced capacity for stomach colonization. These mutants are sensitive to DNA damaging agents and have reduced frequencies of apparent gene conversion between homologous genes encoding outer membrane proteins. Our results reveal requirements for double-strand break repair and recombination during both acute and chronic phases of H. pylori stomach infection.

Original languageEnglish (US)
Pages (from-to)994-1007
Number of pages14
JournalMolecular Microbiology
Volume69
Issue number4
DOIs
StatePublished - Aug 2008

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Helicobacter pylori
DNA Repair
Genetic Recombination
Stomach
Survival
Exodeoxyribonuclease V
Escherichia coli
Genes
Recombinational DNA Repair
Gene Conversion
DNA Breaks
Bacterial Proteins
DNA Adducts
Homologous Recombination
Infection
DNA Damage
Reactive Oxygen Species
Membrane Proteins
Adenosine Triphosphate
Inflammation

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization. / Amundsen, Susan K.; Fero, Jutta; Hansen, Lori M.; Cromie, Gareth A.; Solnick, Jay V; Smith, Gerald R.; Salama, Nina R.

In: Molecular Microbiology, Vol. 69, No. 4, 08.2008, p. 994-1007.

Research output: Contribution to journalArticle

Amundsen, Susan K. ; Fero, Jutta ; Hansen, Lori M. ; Cromie, Gareth A. ; Solnick, Jay V ; Smith, Gerald R. ; Salama, Nina R. / Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization. In: Molecular Microbiology. 2008 ; Vol. 69, No. 4. pp. 994-1007.
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