Helicobacter hepaticus increases intestinal injury in a rat model of necrotizing enterocolitis

Katerina Dvorak, Christine F. Coursodon-Boyiddle, Chelsea L. Snarrenberg, Anchasa Kananurak, Mark Underwood, Bohuslav Dvorak

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Enterohepatic helicobacter species (EHS) infect the intestinal tract and biliary tree, triggering intestinal and hepatic disorders. Helicobacter hepaticus, the prototypic murine EHS, is also associated with inflammation. Necrotizing enterocolitis (NEC) is a devastating disease of premature infants. The cause of NEC is not fully understood, but anomalies of bacterial colonization (dysbiosis) are thought to play an important role in disease onset. To evaluate the effect of H. hepaticus infection on the development of NEC, premature formula-fed rats were kept either in H. hepaticus-free conditions or colonized with H. hepaticus; both groups were exposed to asphyxia and cold stress. The incidence of NEC, expression of Toll-like receptors (TLRs), production of cytokines and mucins, and presence of autophagy regulators were evaluated at the site of injury. H. hepaticus infection increased the incidence of NEC from 39 to 71% and significantly increased levels of TLR4 receptor, expression of proinflammatory cytokines CXCL1, IL-1β, IL-12, and IL-23, and altered activation of autophagy. H. hepaticus induces inflammation and increases the incidence and severity of experimental NEC; this is consistent with observations in neonates of blooms of proinflammatory microbes just before the onset of NEC. Future studies using rodent NEC models should include testing for H. hepaticus infection. Further studies in neonates of early identification and/or diminution of proinflammatory microbes may be beneficial in decreasing the incidence of NEC.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume305
Issue number8
DOIs
StatePublished - Oct 15 2013

Fingerprint

Helicobacter hepaticus
Necrotizing Enterocolitis
Wounds and Injuries
Helicobacter Infections
Helicobacter
Incidence
Autophagy
Infant, Premature, Diseases
Dysbiosis
Cytokines
Inflammation
Interleukin-23
Toll-Like Receptor 4
Toll-Like Receptors
Asphyxia
Biliary Tract
Mucins
Interleukin-12
Interleukin-1
Rodentia

Keywords

  • Autophagy
  • Cytokines
  • Enteral bacteria
  • Mucosal inflammation

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology (medical)
  • Physiology
  • Hepatology

Cite this

Helicobacter hepaticus increases intestinal injury in a rat model of necrotizing enterocolitis. / Dvorak, Katerina; Coursodon-Boyiddle, Christine F.; Snarrenberg, Chelsea L.; Kananurak, Anchasa; Underwood, Mark; Dvorak, Bohuslav.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 305, No. 8, 15.10.2013.

Research output: Contribution to journalArticle

Dvorak, Katerina ; Coursodon-Boyiddle, Christine F. ; Snarrenberg, Chelsea L. ; Kananurak, Anchasa ; Underwood, Mark ; Dvorak, Bohuslav. / Helicobacter hepaticus increases intestinal injury in a rat model of necrotizing enterocolitis. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2013 ; Vol. 305, No. 8.
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