Helicobacter hepaticus Hh0072 gene encodes a novel α1-3- fucosyltransferase belonging to CAZy GT11 family

Lei Zhang, Kam Lau, Jiansong Cheng, Hai Yu, Yanhong Li, Go Sugiarto, Shengshu Huang, Li Ding, Vireak Thon, Peng G. Wang, Xi Chen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Lewis x (Lex) and sialyl Lewis x (SLex)-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is α1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus α1-3- fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Lex, sialyl Lex and their derivatives. Unlike all other α1-3/4- fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with α1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first α1-3-fucosyltransferase identified in the GT11 family.

Original languageEnglish (US)
Pages (from-to)1077-1088
Number of pages12
JournalGlycobiology
Volume20
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

galactoside 3-fucosyltransferase
Helicobacter hepaticus
Glycosyltransferases
Genes
Cloning
Enzymes
Physiological Phenomena
Oligosaccharides
Amino Acid Sequence Homology
Polysaccharides
Molecular Cloning
Pathologic Processes
Carbohydrates
Derivatives
Antigens
Catalysts
Substrates
Proteins

Keywords

  • cloning
  • fucosyltransferase
  • Helicobacter hepaticus
  • Lewis x
  • sialyl Lewis x

ASJC Scopus subject areas

  • Biochemistry

Cite this

Helicobacter hepaticus Hh0072 gene encodes a novel α1-3- fucosyltransferase belonging to CAZy GT11 family. / Zhang, Lei; Lau, Kam; Cheng, Jiansong; Yu, Hai; Li, Yanhong; Sugiarto, Go; Huang, Shengshu; Ding, Li; Thon, Vireak; Wang, Peng G.; Chen, Xi.

In: Glycobiology, Vol. 20, No. 9, 09.2010, p. 1077-1088.

Research output: Contribution to journalArticle

Zhang, L, Lau, K, Cheng, J, Yu, H, Li, Y, Sugiarto, G, Huang, S, Ding, L, Thon, V, Wang, PG & Chen, X 2010, 'Helicobacter hepaticus Hh0072 gene encodes a novel α1-3- fucosyltransferase belonging to CAZy GT11 family', Glycobiology, vol. 20, no. 9, pp. 1077-1088. https://doi.org/10.1093/glycob/cwq068
Zhang, Lei ; Lau, Kam ; Cheng, Jiansong ; Yu, Hai ; Li, Yanhong ; Sugiarto, Go ; Huang, Shengshu ; Ding, Li ; Thon, Vireak ; Wang, Peng G. ; Chen, Xi. / Helicobacter hepaticus Hh0072 gene encodes a novel α1-3- fucosyltransferase belonging to CAZy GT11 family. In: Glycobiology. 2010 ; Vol. 20, No. 9. pp. 1077-1088.
@article{5f65056baf4d4e109f0acb38ca4eb6e4,
title = "Helicobacter hepaticus Hh0072 gene encodes a novel α1-3- fucosyltransferase belonging to CAZy GT11 family",
abstract = "Lewis x (Lex) and sialyl Lewis x (SLex)-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is α1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus α1-3- fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Lex, sialyl Lex and their derivatives. Unlike all other α1-3/4- fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with α1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first α1-3-fucosyltransferase identified in the GT11 family.",
keywords = "cloning, fucosyltransferase, Helicobacter hepaticus, Lewis x, sialyl Lewis x",
author = "Lei Zhang and Kam Lau and Jiansong Cheng and Hai Yu and Yanhong Li and Go Sugiarto and Shengshu Huang and Li Ding and Vireak Thon and Wang, {Peng G.} and Xi Chen",
year = "2010",
month = "9",
doi = "10.1093/glycob/cwq068",
language = "English (US)",
volume = "20",
pages = "1077--1088",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Helicobacter hepaticus Hh0072 gene encodes a novel α1-3- fucosyltransferase belonging to CAZy GT11 family

AU - Zhang, Lei

AU - Lau, Kam

AU - Cheng, Jiansong

AU - Yu, Hai

AU - Li, Yanhong

AU - Sugiarto, Go

AU - Huang, Shengshu

AU - Ding, Li

AU - Thon, Vireak

AU - Wang, Peng G.

AU - Chen, Xi

PY - 2010/9

Y1 - 2010/9

N2 - Lewis x (Lex) and sialyl Lewis x (SLex)-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is α1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus α1-3- fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Lex, sialyl Lex and their derivatives. Unlike all other α1-3/4- fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with α1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first α1-3-fucosyltransferase identified in the GT11 family.

AB - Lewis x (Lex) and sialyl Lewis x (SLex)-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is α1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus α1-3- fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Lex, sialyl Lex and their derivatives. Unlike all other α1-3/4- fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with α1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first α1-3-fucosyltransferase identified in the GT11 family.

KW - cloning

KW - fucosyltransferase

KW - Helicobacter hepaticus

KW - Lewis x

KW - sialyl Lewis x

UR - http://www.scopus.com/inward/record.url?scp=77955459001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955459001&partnerID=8YFLogxK

U2 - 10.1093/glycob/cwq068

DO - 10.1093/glycob/cwq068

M3 - Article

VL - 20

SP - 1077

EP - 1088

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 9

ER -