Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis

J. G. Fox, R. Drolet, Robert Higgins, S. Messier, L. Yan, B. E. Coleman, B. J. Paster, F. E. Dewhirst

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

On the basis of biochemical, phenotypic, and 16S rRNA analysis, a novel gram-negative bacterium, isolated from normal and diarrheic dogs as well as humans with gastroenteritis, has been recently named Helicobacter canis. A 2- month-old female crossbred puppy was submitted to necropsy with a history of weakness and vomiting for several hours prior to death. The liver had multiple and slightly irregular yellowish foci up to 1.5 cm in diameter. Histologically, the liver parenchyma contained randomly distributed, occasionally coalescing hepatocellular necrosis, often accompanied by large numbers of mononuclear cells and neutrophils. Sections of liver stained by the Warthin-Starry silver impregnation technique revealed spiral- to curve- shaped bacteria predominantly located in bile canaliculi and occasionally in bile ducts. Aerobic culture of liver was negative, whereas small colonies were noted on Campylobacter selective media after 5 days of microaerobic incubation. The bacteria were gram negative and oxidase positive but catalase, urease, and indoxyl acetate negative; nitrate was not reduced to nitrite, and the organism did not hydrolyze hippurate. The bacteria were also resistant to 1.5% bile. Electron microscopy revealed spiral-shaped bacteria with bipolar sheathed flagella. By 16S rRNA analysis, the organism was determined to be H. canis. This is the first observation of H. canis in active hepatitis in a dog and correlates with recent findings of Helicobacter hepaticus- and Helicobacter bilis-related hepatic disease in mice. Further studies are clearly warranted to ascertain whether H. canis-associated hepatitis is more widespread in canines as well as a cause of previously classified idiopathic liver disease in humans.

Original languageEnglish (US)
Pages (from-to)2479-2482
Number of pages4
JournalJournal of Clinical Microbiology
Volume34
Issue number10
StatePublished - Oct 1 1996
Externally publishedYes

Fingerprint

Helicobacter
Hepatitis
Dogs
Liver
Gram-Negative Bacteria
Bacteria
Helicobacter hepaticus
Bile Canaliculi
Campylobacter
Flagella
Urease
Gastroenteritis
Nitrites
Bile Ducts
Silver
Bile
Nitrates
Catalase
Vomiting
Canidae

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Fox, J. G., Drolet, R., Higgins, R., Messier, S., Yan, L., Coleman, B. E., ... Dewhirst, F. E. (1996). Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis. Journal of Clinical Microbiology, 34(10), 2479-2482.

Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis. / Fox, J. G.; Drolet, R.; Higgins, Robert; Messier, S.; Yan, L.; Coleman, B. E.; Paster, B. J.; Dewhirst, F. E.

In: Journal of Clinical Microbiology, Vol. 34, No. 10, 01.10.1996, p. 2479-2482.

Research output: Contribution to journalArticle

Fox, JG, Drolet, R, Higgins, R, Messier, S, Yan, L, Coleman, BE, Paster, BJ & Dewhirst, FE 1996, 'Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis', Journal of Clinical Microbiology, vol. 34, no. 10, pp. 2479-2482.
Fox JG, Drolet R, Higgins R, Messier S, Yan L, Coleman BE et al. Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis. Journal of Clinical Microbiology. 1996 Oct 1;34(10):2479-2482.
Fox, J. G. ; Drolet, R. ; Higgins, Robert ; Messier, S. ; Yan, L. ; Coleman, B. E. ; Paster, B. J. ; Dewhirst, F. E. / Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 10. pp. 2479-2482.
@article{323626ea0b73450e81279cd90a98f162,
title = "Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis",
abstract = "On the basis of biochemical, phenotypic, and 16S rRNA analysis, a novel gram-negative bacterium, isolated from normal and diarrheic dogs as well as humans with gastroenteritis, has been recently named Helicobacter canis. A 2- month-old female crossbred puppy was submitted to necropsy with a history of weakness and vomiting for several hours prior to death. The liver had multiple and slightly irregular yellowish foci up to 1.5 cm in diameter. Histologically, the liver parenchyma contained randomly distributed, occasionally coalescing hepatocellular necrosis, often accompanied by large numbers of mononuclear cells and neutrophils. Sections of liver stained by the Warthin-Starry silver impregnation technique revealed spiral- to curve- shaped bacteria predominantly located in bile canaliculi and occasionally in bile ducts. Aerobic culture of liver was negative, whereas small colonies were noted on Campylobacter selective media after 5 days of microaerobic incubation. The bacteria were gram negative and oxidase positive but catalase, urease, and indoxyl acetate negative; nitrate was not reduced to nitrite, and the organism did not hydrolyze hippurate. The bacteria were also resistant to 1.5{\%} bile. Electron microscopy revealed spiral-shaped bacteria with bipolar sheathed flagella. By 16S rRNA analysis, the organism was determined to be H. canis. This is the first observation of H. canis in active hepatitis in a dog and correlates with recent findings of Helicobacter hepaticus- and Helicobacter bilis-related hepatic disease in mice. Further studies are clearly warranted to ascertain whether H. canis-associated hepatitis is more widespread in canines as well as a cause of previously classified idiopathic liver disease in humans.",
author = "Fox, {J. G.} and R. Drolet and Robert Higgins and S. Messier and L. Yan and Coleman, {B. E.} and Paster, {B. J.} and Dewhirst, {F. E.}",
year = "1996",
month = "10",
day = "1",
language = "English (US)",
volume = "34",
pages = "2479--2482",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - Helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis

AU - Fox, J. G.

AU - Drolet, R.

AU - Higgins, Robert

AU - Messier, S.

AU - Yan, L.

AU - Coleman, B. E.

AU - Paster, B. J.

AU - Dewhirst, F. E.

PY - 1996/10/1

Y1 - 1996/10/1

N2 - On the basis of biochemical, phenotypic, and 16S rRNA analysis, a novel gram-negative bacterium, isolated from normal and diarrheic dogs as well as humans with gastroenteritis, has been recently named Helicobacter canis. A 2- month-old female crossbred puppy was submitted to necropsy with a history of weakness and vomiting for several hours prior to death. The liver had multiple and slightly irregular yellowish foci up to 1.5 cm in diameter. Histologically, the liver parenchyma contained randomly distributed, occasionally coalescing hepatocellular necrosis, often accompanied by large numbers of mononuclear cells and neutrophils. Sections of liver stained by the Warthin-Starry silver impregnation technique revealed spiral- to curve- shaped bacteria predominantly located in bile canaliculi and occasionally in bile ducts. Aerobic culture of liver was negative, whereas small colonies were noted on Campylobacter selective media after 5 days of microaerobic incubation. The bacteria were gram negative and oxidase positive but catalase, urease, and indoxyl acetate negative; nitrate was not reduced to nitrite, and the organism did not hydrolyze hippurate. The bacteria were also resistant to 1.5% bile. Electron microscopy revealed spiral-shaped bacteria with bipolar sheathed flagella. By 16S rRNA analysis, the organism was determined to be H. canis. This is the first observation of H. canis in active hepatitis in a dog and correlates with recent findings of Helicobacter hepaticus- and Helicobacter bilis-related hepatic disease in mice. Further studies are clearly warranted to ascertain whether H. canis-associated hepatitis is more widespread in canines as well as a cause of previously classified idiopathic liver disease in humans.

AB - On the basis of biochemical, phenotypic, and 16S rRNA analysis, a novel gram-negative bacterium, isolated from normal and diarrheic dogs as well as humans with gastroenteritis, has been recently named Helicobacter canis. A 2- month-old female crossbred puppy was submitted to necropsy with a history of weakness and vomiting for several hours prior to death. The liver had multiple and slightly irregular yellowish foci up to 1.5 cm in diameter. Histologically, the liver parenchyma contained randomly distributed, occasionally coalescing hepatocellular necrosis, often accompanied by large numbers of mononuclear cells and neutrophils. Sections of liver stained by the Warthin-Starry silver impregnation technique revealed spiral- to curve- shaped bacteria predominantly located in bile canaliculi and occasionally in bile ducts. Aerobic culture of liver was negative, whereas small colonies were noted on Campylobacter selective media after 5 days of microaerobic incubation. The bacteria were gram negative and oxidase positive but catalase, urease, and indoxyl acetate negative; nitrate was not reduced to nitrite, and the organism did not hydrolyze hippurate. The bacteria were also resistant to 1.5% bile. Electron microscopy revealed spiral-shaped bacteria with bipolar sheathed flagella. By 16S rRNA analysis, the organism was determined to be H. canis. This is the first observation of H. canis in active hepatitis in a dog and correlates with recent findings of Helicobacter hepaticus- and Helicobacter bilis-related hepatic disease in mice. Further studies are clearly warranted to ascertain whether H. canis-associated hepatitis is more widespread in canines as well as a cause of previously classified idiopathic liver disease in humans.

UR - http://www.scopus.com/inward/record.url?scp=0029821099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029821099&partnerID=8YFLogxK

M3 - Article

C2 - 8880504

AN - SCOPUS:0029821099

VL - 34

SP - 2479

EP - 2482

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 10

ER -