Heavy and light chains of a mouse monoclonal autoantibody express the same idiotype

M. Zanetti, Fu-Tong Liu, J. Rogers, D. H. Katz

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20 Scopus citations

Abstract

We report on the detection of the same idiotype (Id62) on the heavy and light chains of a mouse monoclonal autoantibody to thyroglobulin. This result was obtained by a simple, sensitive, and reproducible technique, namely immunoblot on nitrocellulose paper of electrophoretically separated heavy and light chains by using a panel of 125I-labeled anti-idiotype probes. These included polyclonal heterologous and syngeneic affinity-purified antibodies and a syngeneic monoclonal antibody. The immunoblot findings were confirmed through the solid-phase radioimmunoassay binding of the anti-idiotype probes to isolated H and L polypeptide chains prepared by conventional chromatography methods. Because anti-idiotype probes define a putative regulatory idiotype of the BALB/c autoimmune response to thyroglobulin, it is suggested that the simultaneous presence of the same idiotype on H and L chains may confer to antibody regulatory properties within the idiotypic network. The significance of the existence of the same idiotype on H and L chains and the implication of this finding for the regulation of the immune response to self antigens are discussed.

Original languageEnglish (US)
Pages (from-to)1245-1251
Number of pages7
JournalJournal of Immunology
Volume135
Issue number2
StatePublished - 1985

ASJC Scopus subject areas

  • Immunology

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    Zanetti, M., Liu, F-T., Rogers, J., & Katz, D. H. (1985). Heavy and light chains of a mouse monoclonal autoantibody express the same idiotype. Journal of Immunology, 135(2), 1245-1251.