Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication

Ziqing Wang, Yi Luo, Qiujia Shao, Ballington L. Kinlock, Chenliang Wang, James Hildreth, Hua Xie, Bindong Liu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Although most human immunodeficiency virus type 1 (HIV-1) cases worldwide are transmitted through mucosal surfaces, transmission through the oral mucosal surface is a rare event. More than 700 bacterial species have been detected in the oral cavity. Despite great efforts to discover oral inhibitors of HIV, little information is available concerning the anti-HIV activity of oral bacterial components. Here we show that a molecule from an oral commensal bacterium, Streptococcus cristatus CC5A can induce expression of APOBEC3G (A3G) and APOBEC3F (A3F) and inhibit HIV-1 replication in THP-1 cells. We show by qRT-PCR that expression levels of A3G and A3F increase in a dose-dependent manner in the presence of a CC5A extract, as does A3G protein levels by Western blot assay. In addition, when the human monocytic cell line THP-1 was treated with CC5A extract, the replication of HIV-1 IIIB was significantly suppressed compared with IIIB replication in untreated THP-1 cells. Knock down of A3G expression in THP-1 cells compromised the ability of CC5A to inhibit HIV-1 IIIB infectivity. Furthermore, SupT1 cells infected with virus produced from CC5A extract-treated THP-1 cells replicated virus with a higher G to A hypermutation rate (a known consequence of A3G activity) than virus used from untreated THP-1 cells. This suggests that S. cristatus CC5A contains a molecule that induces A3G/F expression and thereby inhibits HIV replication. These findings might lead to the discovery of a novel anti-HIV/AIDS therapeutic.

Original languageEnglish (US)
Article numbere106078
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 28 2014

Fingerprint

Streptococcus cristatus
Virus Replication
Human immunodeficiency virus 1
Streptococcus
Viruses
HIV-1
Hot Temperature
mouth
heat
Molecules
HIV
cells
viruses
extracts
Mouth
Acquired Immunodeficiency Syndrome
Western blotting
pathogenicity
Western Blotting
cell lines

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication. / Wang, Ziqing; Luo, Yi; Shao, Qiujia; Kinlock, Ballington L.; Wang, Chenliang; Hildreth, James; Xie, Hua; Liu, Bindong.

In: PLoS One, Vol. 9, No. 8, e106078, 28.08.2014.

Research output: Contribution to journalArticle

Wang, Ziqing ; Luo, Yi ; Shao, Qiujia ; Kinlock, Ballington L. ; Wang, Chenliang ; Hildreth, James ; Xie, Hua ; Liu, Bindong. / Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication. In: PLoS One. 2014 ; Vol. 9, No. 8.
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