Heat shock-induced activation of stress MAP kinase is regulated by threonine-and tyrosine-specific phosphatases

Aaron Ngocky Nguyen, Kazuhiro Shiozaki

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

In eukaryotic species from yeast to human, stress-activated protein kinases (SAPKs), members of a MAP kinase (MAPK) subfamily, regulate the transcriptional response to various environmental stress. It is poorly understood how diverse forms of stress are sensed and transmitted to SAPKs. Here, we report the heat shock regulation of the fission yeast Spc1 SAPK, a homolog of human p38 and budding yeast Hog1p. Although osmostress and oxidative stress induce strong activation of the Wis1 MAPK kinase (MEK), which activates Spc1 through Thr-171/Tyr-173 phosphorylation, activation of Wis1 upon heat shock is relatively weak and transient. However, in heat- shocked cells, Pyp1, the major tyrosine phosphatase that dephosphorylates and inactivates Spc1, is inhibited for its interaction with Spc1, which leads to strong activation of Spc1. Subsequently, Spc1 activity is rapidly attenuated by Thr-171 dephosphorylation, whereas Tyr-173 remains phosphorylated. Thr- 171 dephosphorylation is compromised in a strain lacking functional type 2C serine/threonine phosphatases (PP2C), Ptc1 and Ptc3. Moreover, Ptc1 and Ptc3 can dephosphorylate Thr-171 of Spc1 both in vivo and in vitro. These observations strongly suggest that PP2C enzymes play an important role in the attenuation of Spc1 activity in heat-shocked cells. Thus, transient activation of Spc1 upon heat shock is ensured by differential regulation of threonine and tyrosine phosphorylation.

Original languageEnglish (US)
Pages (from-to)1653-1663
Number of pages11
JournalGenes and Development
Volume13
Issue number13
StatePublished - Jul 1 1999

Fingerprint

Threonine
Phosphoric Monoester Hydrolases
Tyrosine
Shock
Phosphotransferases
Hot Temperature
Heat-Shock Proteins
Protein Kinases
Mitogen-Activated Protein Kinase Kinases
Phosphorylation
Saccharomycetales
Schizosaccharomyces
Phosphoprotein Phosphatases
Oxidative Stress
Yeasts
Enzymes

Keywords

  • Heat shock
  • Protein phosphatase 2C
  • Protein tyrosine phosphatase
  • Schizosaccharomyces pombe
  • Spc1
  • Stress-activated protein kinase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Heat shock-induced activation of stress MAP kinase is regulated by threonine-and tyrosine-specific phosphatases. / Nguyen, Aaron Ngocky; Shiozaki, Kazuhiro.

In: Genes and Development, Vol. 13, No. 13, 01.07.1999, p. 1653-1663.

Research output: Contribution to journalArticle

Nguyen, Aaron Ngocky ; Shiozaki, Kazuhiro. / Heat shock-induced activation of stress MAP kinase is regulated by threonine-and tyrosine-specific phosphatases. In: Genes and Development. 1999 ; Vol. 13, No. 13. pp. 1653-1663.
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