Heat induced collagen denaturation and its effects on bone mechanical integrity

X. Wang, R. A. Bank, X. Shen, J. M. Te Koppele, K. A. Athanasiou, C. M. Agrawal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The present study was performed to examine the effect of collagen denaturation on bone mechanical integrity. To introduce varied degrees of collagen denaturation in situ, bone specimens were heated at temperatures lower, at, and beyond the denaturing temperature of calcified collagen (160 °C). Three point bending tests were performed to assess the mechanical integrity of the bone specimens. The heat-induced collagen denaturation was quantified using a selective digestion technique. The results of this study indicate that in situ denaturation of collagen molecules induced by heating has a significant impact on the strength and toughness, but little effect on the stiffness of bone. This finding experimentally supports the speculation that mineral imparts stiffness to bone, whereas the collagen network sustains its toughness and strength.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages493
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Denaturation
Collagen
Bone
Toughness
Stiffness
Bending tests
Minerals
Hot Temperature
Heating
Temperature
Molecules

ASJC Scopus subject areas

  • Bioengineering

Cite this

Wang, X., Bank, R. A., Shen, X., Te Koppele, J. M., Athanasiou, K. A., & Agrawal, C. M. (1999). Heat induced collagen denaturation and its effects on bone mechanical integrity. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 493). Piscataway, NJ, United States: IEEE.

Heat induced collagen denaturation and its effects on bone mechanical integrity. / Wang, X.; Bank, R. A.; Shen, X.; Te Koppele, J. M.; Athanasiou, K. A.; Agrawal, C. M.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. p. 493.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, X, Bank, RA, Shen, X, Te Koppele, JM, Athanasiou, KA & Agrawal, CM 1999, Heat induced collagen denaturation and its effects on bone mechanical integrity. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, Piscataway, NJ, United States, pp. 493, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Wang X, Bank RA, Shen X, Te Koppele JM, Athanasiou KA, Agrawal CM. Heat induced collagen denaturation and its effects on bone mechanical integrity. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. Piscataway, NJ, United States: IEEE. 1999. p. 493
Wang, X. ; Bank, R. A. ; Shen, X. ; Te Koppele, J. M. ; Athanasiou, K. A. ; Agrawal, C. M. / Heat induced collagen denaturation and its effects on bone mechanical integrity. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. pp. 493
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