Heart changes in 17-day-old fetuses of diabetic ICR (Institute of Cancer Research) mothers: Improvement with maternal immune stimulation

Juan claudio Gutierrez, Mary Renee Prater, Theresa C. Hrubec, Bonnie J. Smith, Larry E. Freeman, Steven D. Holladay

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Maternal diabetes mellitus is associated with increased fetal teratogenesis, including cardiovascular defects. Non-specific maternal immune stimulation with Freund's complete adjuvant (FCA) or interferon gamma (IFNγ) has been associated with protection against birth malformations. Using a diabetic mouse model, late-gestation fetal heart and great vessel morphology were analyzed. Four groups of mice were used: non-diabetic females as a control group, hyperglycemic females induced by streptozotocin as a diabetic group, and diabetic females injected either with FCA or IFNγ. At day 17 of gestation, females were euthanized and one fetus was arbitrarily selected per litter for fixation and sectioning. Treatment-induced changes in cardiac development were assessed from digital images of serial sections taken at standardized levels in the thorax. One-way parametric and non-parametric ANOVA and ordinal logistic regression were performed to compare the difference among groups (P < 0.05). Maternal hyperglycemia altered morphology of the late-gestation fetal mouse heart by causing ventricular chamber dilation, sectional myocardial reduction, and an increase in transversal aortic area. FCA protected the fetal heart from cavitary dilation in diabetic mothers. FCA and IFNγ protected the fetal heart against reduction of myocardial area, and ascending thoracic aorta dilation. Consequences of late gestation heart chamber dilation and myocardial reduction are not yet known. Maternal immune stimulation partially protected against these developmental defects by mechanisms that remain unclear.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalCongenital Anomalies
Volume49
Issue number1
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

Fetal Heart
Freund's Adjuvant
Dilatation
Fetus
Mothers
Interferon-gamma
Pregnancy
Neoplasms
Teratogenesis
Streptozocin
Thoracic Aorta
Hyperglycemia
Aorta
Diabetes Mellitus
Analysis of Variance
Thorax
Logistic Models
Parturition
Control Groups

Keywords

  • Cardiovascular malformation
  • Diabetes and pregnancy
  • Type 1 diabetes

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health
  • Embryology

Cite this

Heart changes in 17-day-old fetuses of diabetic ICR (Institute of Cancer Research) mothers : Improvement with maternal immune stimulation. / Gutierrez, Juan claudio; Prater, Mary Renee; Hrubec, Theresa C.; Smith, Bonnie J.; Freeman, Larry E.; Holladay, Steven D.

In: Congenital Anomalies, Vol. 49, No. 1, 03.2009, p. 1-7.

Research output: Contribution to journalArticle

Gutierrez, Juan claudio ; Prater, Mary Renee ; Hrubec, Theresa C. ; Smith, Bonnie J. ; Freeman, Larry E. ; Holladay, Steven D. / Heart changes in 17-day-old fetuses of diabetic ICR (Institute of Cancer Research) mothers : Improvement with maternal immune stimulation. In: Congenital Anomalies. 2009 ; Vol. 49, No. 1. pp. 1-7.
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